Gene Ontology annotation based on Enzyme Commission mapping

Combined Automated Annotation using Multiple IEA Methods

Characterization of TCL, a new GTPase of the rho family related to TC10 andCcdc42.

Full-length human TCL/RHOJ has intrinsic GTP hydrolysis activity, distinguishing it from GTPase-deficient Rho-family proteins.

"In vitro, TCL shows rapid GDP/GTP exchange and displays higher GTP dissociation and hydolysis rates than TC10."

TCL/RhoJ Plasma Membrane Localization and Nucleotide Exchange Is Coordinately Regulated by Amino Acids within the N Terminus and a Distal Loop Region.

Human RHOJ nucleotide exchange and membrane localization depend on sequence regions including a distal loop; this supports caution for an altered C-terminal product.

"Chimeras of TCL and TC10 revealed amino acids 121-129 of TCL contributed to the differences in nucleotide loading."

file:human/RHOJ/RHOJ-bioinformatics/RESULTS.md

Exact RHOJ product-to-reference sequence mapping

file:human/RHOJ/RHOJ-protnlm-source.json

Frozen exact-input ProtNLM output and UniProt sequence for G3V4H1