Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Protein O-mannosylation is necessary for normal embryonic development in zebrafish.
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Zebrafish Pomt1 and Pomt2 are both required for full protein O-mannosyltransferase activity when reconstituted in HEK293T cells.
"only when both zPOMT1 and zPOMT2 were expressed in human embryonic kidney 293T cells were high levels of protein O-mannosyltransferase activity detected, indicating that both zPOMT1 and zPOMT2 were required for full enzymatic activity"
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Zebrafish and human POMT subunits are interchangeable, indicating a conserved heteromeric enzyme.
"either heterologous combination, zPOMT1 and human POMT2 (hPOMT2) or hPOMT1 and zPOMT2, resulted in enzymatic activity in cultured cells"
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Knockdown of pomt2 abolishes glycosylated alpha-dystroglycan (IIH6 epitope) in embryos and causes body-axis, pericardial and eye-pigmentation defects.
"Injection of antisense morpholino oligonucleotides of zPOMT1 and zPOMT2 resulted in several severe phenotypes-including bended body, edematous pericaridium and abnormal eye pigmentation."
Deletion of POMT2 in Zebrafish Causes Degeneration of Photoreceptors.
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A CRISPR pomt2 null allele abolishes matriglycan on alpha-dystroglycan and EYS binding.
"pomt2 mutation resulted in a loss of matriglycan and abolished binding of EYS protein to α-dystroglycan"
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pomt2 mutants show hydrocephalus, cerebellar hypoplasia and muscular dystrophy, the dystroglycanopathy triad.
"Mutant zebrafish presented with hydrocephalus and hypoplasia of the cerebellum, as well as muscular dystrophy."
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Photoreceptor development is normal but late-onset rod and cone degeneration follows loss of EYS from the connecting cilium region.
"While photoreceptor development was not affected in pomt2 mutant zebrafish, photoreceptor density was significantly reduced by 6-mpf. Apoptosis of both cone and rod photoreceptors in the pomt2 mutant retina was significantly increased."
UniProtKB entry F1Q8R9 for Danio rerio pomt2
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Pomt2 transfers mannose to Ser/Thr hydroxyls and requires co-expression with Pomt1 for activity.
"FUNCTION: Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. Coexpression of both POMT1 and POMT2 is necessary for enzyme activity, expression of either POMT1 or POMT2 alone is insufficient."
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The protein is a multi-pass ER membrane protein (location transferred from human POMT2).
"SUBCELLULAR LOCATION: Endoplasmic reticulum membrane"
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Morpholino knockdown severely reduces alpha-dystroglycan glycosylation.
"Glycosylation of alpha-dystroglycan (dag1) is severely reduced."