GO_REF:0000118

TreeGrafter-generated GO annotations

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

GO:0008479

tRNA-guanosine(34) queuine transglycosylase activity

The current GO definition specifies free-queuine insertion and cross-references RHEA:16633.

file:rules/arba/_interpro2go.txt

InterPro2GO mappings

The GOC-maintained mapping assigns bacterial Tgt family IPR004803 to GO:0008479.

file:rules/unirule/UR000079182/UR000079182.json

UniRule UR000079182 for bacterial Tgt

HAMAP MF_00168 associates GO:0008479 with EC 2.4.2.29 and the bacterial preQ1 exchange reaction RHEA:24104.

PMID:40703034

Limitations of current machine learning models in predicting enzymatic functions for uncharacterized proteins.

Experimental genetics assigns preQ1 insertion in tRNA to bacterial Tgt.

"This activity is catalyzed by Tgt/b0406, and the mutant does not insert preQ1 in tRNA"

file:PSEPK/tgt/tgt-uniprot.txt

UniProtKB entry for PSEPK tgt

P. putida Tgt exchanges guanine 34 for preQ1 in GUN-anticodon tRNAs.

"queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34"

file:PSEPK/tgt/tgt-deep-research-openscientist.md

OpenScientist functional-annotation report for tgt

The report corroborates Q88PL7 as a bacterial preQ1-inserting tRNA-guanine transglycosylase and explicitly finds no direct P. putida-specific experimental characterization.

file:interpro/panther/PTHR46499/PTHR46499-metadata.yaml

PANTHER family metadata for PTHR46499

Q88PL7 belongs to the queuine tRNA-ribosyltransferase family.

"name: QUEUINE TRNA-RIBOSYLTRANSFERASE"

file:interpro/panther/PTHR46499/PTHR46499-entries.csv

PANTHER family members for PTHR46499

Q88PL7 is assigned to the Tgt subfamily.

"Q88PL7,Queuine tRNA-ribosyltransferase"