Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

Annotation inferences using phylogenetic trees

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

Gene Ontology annotation based on curation of immunofluorescence data

Subcellular localization of fukutin and fukutin-related protein in muscle cells.

Fukutin-related protein localizes to the Golgi apparatus and mutations lead to mislocalization in muscle in vivo.

Mutations alter secretion of fukutin-related protein.

Fukutin-related protein resides in the Golgi cisternae of skeletal muscle fibres and forms disulfide-linked homodimers via an N-terminal interaction.

The glucuronyltransferase B4GAT1 is required for initiation of LARGE-mediated α-dystroglycan functional glycosylation.

Identification of a Post-translational Modification with Ribitol-Phosphate and Its Defect in Muscular Dystrophy.

ISPD produces CDP-ribitol used by FKTN and FKRP to transfer ribitol phosphate onto α-dystroglycan.

Direct Mapping of Additional Modifications on Phosphorylated O-glycans of α-Dystroglycan by Mass Spectrometry Analysis in Conjunction with Knocking Out of Causative Genes for Dystroglycanopathy.

Cell endogenous activities of fukutin and FKRP coexist with the ribitol xylosyltransferase, TMEM5.

Crystal structures of fukutin-related protein (FKRP), a ribitol-phosphate transferase related to muscular dystrophy.

FKTN transfers RboP to GalNAc-GlcNAc-ManP-DAG1

FKRP dimer transfers RboP to matriglycan chain

RXYLT1 transfers initial Xyl to matriglycan chain

POMGNT1 catalyzes FKRP:FKTN:RXYLT1 formation

Fukutin-related protein associates with the sarcolemmal dystrophin-glycoprotein complex.

CDP-glycerol inhibits the synthesis of the functional O-mannosyl glycan of α-dystroglycan.

FKRP source evidence, context coverage and experimental boundaries