Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Combined Automated Annotation using Multiple IEA Methods
The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification.
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Immunopurification of human Complex I followed by MS identified NDUFA8 among the resolved Complex I polypeptides, supporting its membership in the complex.
"we can resolve and identify the human homologues of 42 polypeptides detected so far in the more extensively studied beef heart complex I"
NDUFB7 and NDUFA8 are located at the intermembrane surface of complex I.
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NDUFA8 (with NDUFB7) is localized at the intermembrane-space surface of Complex I; both are CX9C-containing subunits with probable intramolecular disulfide bonds that stabilize the membrane domain of the complex.
"We experimentally confirm the localization of the latter two, while our data are consistent with disulfide bridges in NDUFA8. We propose these subunits stabilize the membrane domain of complex I."
KIF14 negatively regulates Rap1a-Radil signaling during breast cancer progression.
Protein import and oxidative folding in the mitochondrial intermembrane space of intact mammalian cells.
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Characterizes the MIA40/ALR disulfide-relay pathway that imports and oxidatively folds CX9C proteins in the mammalian intermembrane space, the pathway relevant to NDUFA8 import and disulfide-bond formation.
"Oxidation of cysteine residues to disulfides drives import of many proteins into the intermembrane space of mitochondria."
Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function.
Accessory subunits are integral for assembly and function of human mitochondrial complex I.
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Systematic CRISPR knockout of each Complex I accessory subunit shows most are required for assembly of a functional complex, and loss of a subunit destabilizes other subunits in the same structural module; establishes the structural/assembly role of accessory subunits including NDUFA8.
"We show that 25 subunits are strictly required for assembly of a functional complex and 1 subunit is essential for cell viability."
Architecture of Human Mitochondrial Respiratory Megacomplex I(2)III(2)IV(2).
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Cryo-EM of the human respiratory megacomplex resolves individual Complex I subunits, providing structural placement of NDUFA8 within Complex I at the inner membrane.
"The structure not only reveals the precise assignment of individual subunits of human CI and CIII"
Assembly of mammalian oxidative phosphorylation complexes I-V and supercomplexes.
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Review of OXPHOS complex and supercomplex assembly; distinguishes core catalytic proteins from supernumerary subunits that play roles in assembly, regulation and stability.
"The human enzymes comprise core proteins, performing the catalytic activities, and a large number of 'supernumerary' subunits that play essential roles in assembly, regulation and stability."
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
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High-confidence human mitochondrial proteome dataset in which NDUFA8 is detected as a mitochondrial protein (basis for the HTP mitochondrion localization).
"Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context"
The nuclear-encoded human NADH:ubiquinone oxidoreductase NDUFA8 subunit: cDNA cloning, chromosomal localization, tissue distribution, and mutation detection in complex-I-deficient patients.
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Original cloning of human NDUFA8 (172 aa, ~20.1 kDa, chromosome 9); contains a conserved cysteine motif; highest expression in heart and skeletal muscle; bovine ortholog is PGIV.
"Both human and bovine NDUFA8 contain a conserved cysteine motif."
cDNA of eight nuclear encoded subunits of NADH:ubiquinone oxidoreductase: human complex I cDNA characterization completed.
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Reports cDNAs of nuclear-encoded Complex I subunits and describes Complex I's function in electron transport from NADH to ubiquinone coupled to proton translocation.
"Its main function is the transport of electrons from NADH to ubiquinone, which is accompanied by translocation of protons from the mitochondrial matrix to the intermembrane space."
Complex I oxidises NADH to NAD+, reduces CoQ to CoQH2
Intermediate 1 binds HP subcomplex to form Intermediate 2
Peripheral arm subunits bind the 815kDa complex to form a 980kDa complex
Intermediate 2 binds MT-ND1:NDUFAF5:NDUFAF6 to form a 315kDa subcomplex
The MCIA complex, NDUFAF2-7 all dissociate from the 980kDa complex, resulting in Complex I
ND4, ND5 bind the 550kDa complex to form the 815kDa complex
The 315kDa subcomplex binds the 370kDa subcomplex to form the 550kDa complex