Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
A proteome-scale map of the human interactome network.
A reference map of the human binary protein interactome.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
LOC66273 isoform 2, a novel protein highly expressed in white adipose tissue, induces adipogenesis in 3T3-L1 cells.
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Overexpression of the mouse AAMDC ortholog (LOC66273 isoform 2, "LI2") was sufficient to drive 3T3-L1 preadipocyte differentiation without insulin, acting through CREB phosphorylation and transcriptional activity.
"Our results indicated that LI2 was sufficient to drive preadipocyte differentiation via modulating the phosphorylation level and transcriptional activity of CREB, coincident with expression of several adipogenic regulators and mature adipocyte markers, without insulin treatment."
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Knockdown of the protein caused preadipocyte apoptosis via caspase-3 activation during adipogenesis, giving loss-of-function support alongside the gain-of-function result.
"knockdown of the LI2 protein resulted in preadipocyte apoptosis via caspase-3 activation during adipogenesis"
The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer.
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Ectopic AAMDC expression alone is sufficient to activate AKT signalling and to support estrogen-independent tumour growth, establishing a causal rather than correlative role in PI3K-AKT-mTOR activation.
"Ectopic AAMDC expression is sufficient to activate AKT signaling, resulting in estrogen-independent tumor growth."
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AAMDC controls PI3K-AKT-mTOR signalling, the translation of ATF4 and MYC, and the transcriptional activity of AAMDC-dependent promoters.
"We show that AAMDC controls PI3K-AKT-mTOR signaling, regulating the translation of ATF4 and MYC and modulating the transcriptional activity of AAMDC-dependent promoters."
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AAMDC regulates expression of metabolic enzymes of the one-carbon folate and methionine cycles and of lipid metabolism.
"We uncover that AAMDC regulates the expression of several metabolic enzymes involved in the one-carbon folate and methionine cycles, and lipid metabolism."
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AAMDC interacts with the Rab GTPase-activating protein RABGAP1L, and AAMDC, RABGAP1L and RAB7A colocalise in endolysosomes.
"we provide evidence that AAMDC can interact with the RabGTPase-activating protein RabGAP1L, and that AAMDC, RabGAP1L, and Rab7a colocalize in endolysosomes."
Solanine Represses Gastric Cancer Growth by Mediating Autophagy Through AAMDC/MYC/ATF4/Sesn2 Signaling Pathway.
Is a molecular function assignable to AAMDC from its family?
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Family-wide analysis of Pfam PF04430 (DUF498) run for this review: of 13 reviewed Swiss-Prot members, none carries an experimentally supported molecular function term or a UniProt CATALYTIC ACTIVITY block, and four carry an explicit GO:0003674 ND. The absence of a molecular function for AAMDC is a property of the whole family rather than an oversight on this gene.
"No member of this family carries any experimentally supported molecular function term."
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The one characterised branch of the family is NDUFAF3, an assembly factor for mitochondrial complex I in every organism studied - a non-catalytic protein that binds subunits and helps build a complex. The family signal therefore points away from a hidden enzymatic activity and toward an assembly or chaperone-like role.
"NDUFAF3 is an **assembly factor, not an enzyme**: it has no catalytic activity, it binds subunits and helps build a complex, and it is not part of the finished complex."
Affinage mechanistic annotation for AAMDC (human)
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Machine-fetched Affinage record (trust gates passed) describing AAMDC as an oncogenic PI3K-AKT-mTOR regulator amplified in ER-positive breast cancer that interacts with RABGAP1L and colocalises with RAB7A at endolysosomes, and explicitly noting that the biochemical activity of AAMDC itself has not been characterised.
"Beyond these findings, the biochemical activity of AAMDC itself and the structural basis of its signaling and RabGAP1L interaction have not been characterized in the available corpus."