Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Deep research review of ATP5IF1 gene function (Falcon provider)
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ATP5IF1 encodes mitochondrial ATPase inhibitory factor 1, a reversible pH-sensitive inhibitor of F1Fo-ATP synthase that prevents ATP hydrolysis under low proton motive force conditions.
"IF1 is a reversible, pH-sensitive inhibitor that primarily prevents wasteful ATP hydrolysis when Δψm collapses, but can also inhibit ATP synthesis in some contexts"
Nucleotide sequence of cDNA coding the mitochondrial precursor protein of the ATPase inhibitor from humans.
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Cloned and sequenced the human IF1 cDNA encoding a 106 amino acid precursor protein
"The deduced protein sequence shows that the protein was composed of 106 amino acids and had a molecular weight of 12248"
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Identified the protein as the human homolog of the bovine ATPase inhibitor
"cDNA coding the human homolog of the inhibitor protein was isolated and sequenced"
A functionally active human F1F0 ATPase can be purified by immunocapture from heart tissue and fibroblast cell lines. Subunit structure and activity studies.
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IF1 co-purifies with F1Fo-ATP synthase at pH 6.5 but not at pH 8.0
"IF(1) could be co-isolated with F(1)F(0) when the immunocapture procedure was carried out at pH 6.5 but was absent when the ATP synthase was isolated at pH 8.0"
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Recombinant IF1 inhibits ATP hydrolysis by >90%
"Immunocaptured F(1)F(0) lacking IF(1) could be inhibited by more than 90% by addition of recombinant inhibitor protein"
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IF1 release at pH 8.0 activates ATP hydrolysis >10-fold
"F(1)F(0) containing IF(1) could be activated more than 10-fold by brief exposure to pH 8.0, inducing the release of inhibitor protein"
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IF1 detected by specific antibody in immunocaptured complex from heart mitochondria
"The immunoprecipitated F(1)F(0) contained a full complement of subunits that were identified with specific antibodies against five of the subunits (alpha, beta, OSCP, d, and IF(1))"
An Inhibitor of the F1 subunit of ATP synthase (IF1) modulates the activity of angiostatin on the endothelial cell surface.
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IF1 binds purified F1-ATP synthase and inhibits ATP hydrolysis
"IF1 protein bound to purified F(1) ATP synthase and inhibited F(1)-dependent ATP hydrolysis consistent with its reported activity in studies of mitochondria"
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IF1 inhibits ATP hydrolysis but not ATP synthesis on cell surface
"IF1 inhibited ATP hydrolysis but not ATP synthesis, in contrast to angiostatin, which inhibited both"
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IF1 only slightly inhibits endothelial cell proliferation
"IF1 did not inhibit EC differentiation to form tubes and only slightly inhibited cell proliferation compared with angiostatin"
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IF1 is not an angiostatin mimetic
"We propose that IF1 is not an angiostatin mimetic, but it can serve a protective role for EC in the tumor microenvironment"
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Angiostatin blocks IF1 binding to ATP synthase
"angiostatin blocks IF1 binding to ATP synthase and abolishes its ability to conserve ATP"
Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Regenerative protein thymosin beta-4 is a novel regulator of purinergic signaling.
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IF1 shares amphiphilic alpha-helical structural similarities with beta-thymosins
"in silico we identified common amphiphilic α-helical structural similarities between β-thymosins and the inhibitory factor 1 (IF1), an inhibitor of ATP synthase hydrolysis"
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IF1 is an inhibitor of ATP synthase hydrolysis
"the inhibitory factor 1 (IF1), an inhibitor of ATP synthase hydrolysis"
Mitochondrial Atpif1 regulates haem synthesis in developing erythroblasts.
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Atpif1 regulates heme synthesis by modulating mitochondrial pH and redox potential
"Atpif1 modulates the mitochondrial pH and redox potential, and thus allows Fech to efficiently catalyze the incorporation of iron into PPIX to produce heme"
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Required for FECH to catalyze iron incorporation into protoporphyrin IX
"Loss of Atpif1 allows the mitochondrial pH and, consequently, the redox potential to change to a level that reduces [2Fe-2S] cluster-containing Fech activity, thereby reducing heme synthesis"
Genome-wide RNAi screen identifies ATPase inhibitory factor 1 (ATPIF1) as essential for PARK2 recruitment and mitophagy.
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ATPIF1 is essential for PARK2 translocation to mitochondria
"we identified ATPase inhibitory factor 1 (ATPIF1/IF1) as essential for PARK2 recruitment and mitophagy in cultured cells"
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ATPIF1 promotes mitochondrial depolarization during uncoupling
"During uncoupling, ATPIF1 promotes collapse of ΔΨ and activation of the PINK-PARK2 mitophagy pathway by blocking the ATPase activity of the F 1-Fo ATP synthase"
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ATPIF1 blocks ATPase activity of F1-Fo ATP synthase
"blocking the ATPase activity of the F 1-Fo ATP synthase"
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Restoration of ATPIF1 in Rho0 cells lowers membrane potential and triggers PARK2 recruitment
"Restoration of ATPIF1 in Rho0 cells, which lack mtDNA and a functional electron transport chain, lowers ΔΨ and triggers PARK2 recruitment"
Architecture of the human interactome defines protein communities and disease networks.
Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei.
A reference map of the human binary protein interactome.
OSMR controls glioma stem cell respiration and confers resistance of glioblastoma to ionizing radiation.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Mitochondrial remodeling and ischemic protection by G protein-coupled receptor 35 agonists.
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GPR35 binds ATPIF1 at the outer mitochondrial membrane
"GPR35 activated Gi- and G12/13-coupled signaling and trafficked to the outer mitochondria membrane, where it bound, apparantly indirectly, to ATP synthase inhibitory factor subunit 1 (ATPIF1)"
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ATPIF1-dependent ATP synthase dimerization prevents ATP loss during ischemia
"Activated GPR35, in an ATPIF1-dependent and pertussis toxin-sensitive manner, induced ATP synthase dimerization, which prevented ATP loss upon ischemia"
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Activated GPR35 in pertussis toxin-sensitive manner induces ATP synthase dimerization
"Activated GPR35, in an ATPIF1-dependent and pertussis toxin-sensitive manner, induced ATP synthase dimerization"
Multimodal cell maps as a foundation for structural and functional genomics.