GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

UniProtKB:C5CY00

UniProtKB/Swiss-Prot record C5CY00 (URE1_VARPS) - Urease subunit alpha from Variovorax paradoxus S110

Vapar_4243 encodes the 572-aa catalytic alpha subunit (UreC, EC 3.5.1.5) of cytoplasmic urease in Variovorax paradoxus S110, which forms a (UreABC)3 trimer of heterotrimers and coordinates two Ni(2+) ions per subunit via His139, His141, carboxylated Lys222, His251, His277, and Asp365.

"SUBUNIT: Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme. {ECO:0000255|HAMAP-Rule:MF_01953}."

PMID:21183664

Complete genome sequence of the metabolically versatile plant growth-promoting endophyte Variovorax paradoxus S110.

Complete genome sequence of Variovorax paradoxus S110 identifying Vapar_4243 (ureC) and its metabolic repertoire for plant-associated nitrogen and mineral cycling.

"The complete genome sequence of V. paradoxus S110 is composed of 6,754,997 bp with 6,279 predicted protein-coding sequences within two circular chromosomes."

PMID:20046957

Interplay of metal ions and urease.

Bacterial three-subunit ureases assemble into a (UreABC)3 holoenzyme whose UreC subunit houses a dinuclear nickel metallocenter bridged by a carboxylated lysine residue, matured in vivo by the UreD, UreF, UreG, and UreE accessory proteins.

"The active site of urease contains two nickel atoms bridged by a carboxylated lysine and a solvent molecule, as shown for the K. aerogenes enzyme (Fig. 2).19 In addition, Ni1 is coordinated by two His residues plus a terminal water molecule while Ni2 is coordinated by two His, an Asp, and a terminal solvent."