Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Sorting signals, N-terminal modifications and abundance of the chloroplast proteome.
Quantitative proteomics of a chloroplast SRP54 sorting mutant and its genetic interactions with CLPC1 in Arabidopsis.
Omics-based approaches to methionine side chain elongation in Arabidopsis: characterization of the genes encoding methylthioalkylmalate isomerase and methylthioalkylmalate dehydrogenase.
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atleuc1 knockouts have reduced Met-derived glucosinolates, especially long-chain species; gene named MAM-IL1.
"In this study we found that knocking out either of the candidate genes, AtleuC1 or AtIMD1 , reduced the level of Met-GSLs, indicating that both genes are actually involved in Met-GSL biosynthesis."
Arabidopsis thaliana encodes a bacterial-type heterodimeric isopropylmalate isomerase involved in both Leu biosynthesis and the Met chain elongation pathway of glucosinolate formation.
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IIL1 is the single IPMI large subunit; lsu1 mutants accumulate 2-IPM and Met chain-elongation intermediates and shift aliphatic glucosinolates toward shorter chains, establishing a dual role in Leu and glucosinolate biosynthesis.
"Metabolic profiling of large subunit mutants revealed accumulation of intermediates of both Leu biosynthesis and Met chain elongation, and an altered composition of aliphatic glucosinolates demonstrating the function of this gene in both pathways."
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Small subunits are proposed to determine substrate specificity; the heterodimer is pathway-specific even though the large subunit is not.
"Thus, the resulting IPMI heterodimer is pathway-specific even if the large subunit per se is not."
AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins.
Putative glycosyltransferases and other plant Golgi apparatus proteins are revealed by LOPIT proteomics.
A proteomic strategy for global analysis of plant protein complexes.
The small subunit 1 of the Arabidopsis isopropylmalate isomerase is required for normal growth and development and the early stages of glucosinolate formation.
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IPMI is a heterodimer; IIL1 promoter active in and along the vasculature like SSU2/SSU3.
"The promoter of IPMI LSU1 showed relatively high activity mainly in or along the vasculature, similar to the promoters of IPMI SSU2 and IPMI SSU3"
Protein complex formation in methionine chain-elongation and leucine biosynthesis.
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LeuC interacts with IPMDH1 and IPMDH2 (Y2H, BiFC, Co-IP) but no LeuC-LeuD interaction was detected by Y2H; leuc ipmdh1 double mutant enhances the GSL chain-length phenotype.
"It has been demonstrated that LeuC plays dual roles in both GLS and Leu biosynthesis"
In Arabidopsis thaliana Substrate Recognition and Tissue- as Well as Plastid Type-Specific Expression Define the Roles of Distinct Small Subunits of Isopropylmalate Isomerase.
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The small subunit defines IPMI function; SSU1 serves Leu biosynthesis, SSU2/SSU3 Met chain elongation.
"The function of IPMI is defined by the small subunits."
Functional specification of Arabidopsis isopropylmalate isomerases in glucosinolate and leucine biosynthesis.
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LeuC physically interacts with LeuD proteins forming functional IPMIs in the chloroplast stroma.
"We demonstrate here that AtLeuC physically interacts with AtLeuD proteins to form functional IPMIs. The IPMIs are localized to chloroplast stroma."
A genetic defect caused by a triplet repeat expansion in Arabidopsis thaliana.
Biosynthesis of glucosinolates--gene discovery and beyond.