gpat3 notes

2026-05-09 review notes

Reviewed GOA, UniProt Q6DG38, and PANTHER family cache. The core role is endoplasmic-reticulum glycerol-3-phosphate/acylglycerol-3-phosphate O-acyltransferase activity feeding glycerolipid/triglyceride and CDP-diacylglycerol biosynthesis; generic acyltransferase terms were modified to the specific GPAT activity.

Re-review 2026-09-29

• The previous review cited no primary literature (only UniProt and the deep-research file). Cached and cited the three primary papers on the orthologue: Cao et al. 2006 PMID:17170135, Sukumaran et al. 2009 PMID:19318427 and the Gpat3-null mouse PMID:24714397. No zebrafish-specific enzymology or phenotype paper was found (PubMed "gpat3 AND zebrafish" returns only metabolomics/extract studies).
• GPAT activity (GO:0004366, IEA + 2 ISS) ACCEPT on the strength of PMID:17170135 and PMID:24714397.
• AGPAT activity (GO:0003841, IEA + 2 ISS) changed ACCEPT -> UNDECIDED: the two full-text human papers contradict each other. PMID:19318427 versus PMID:17170135. PMID:19318427 is marked DISPUTED in reference_review. The AGPAT core_functions entry was removed; only the GPAT entry remains.
• GO:0005783 endoplasmic reticulum: new IBA (PTN000551966) and the ISS row both ACCEPT (ISS was KEEP_AS_NON_CORE); the ER-membrane rows now cite the SUBCELLULAR LOCATION line and PMID:17170135.
• New IEA GO:0016747 -> MODIFY to GO:0004366 (like the existing GO:0016746 row). GO:0016024 CDP-diacylglycerol biosynthesis ACCEPT -> KEEP_AS_NON_CORE because the only functional evidence is TAG synthesis PMID:17170135.
• Rewrote description, added reference_review/findings, two questions and one experiment. Validation: 0 errors, 0 warnings.