Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
A distinct type of alcohol dehydrogenase, adh4+, complements ethanol fermentation in an adh1-deficient strain of Schizosaccharomyces pombe.
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Single- and double-gene disruption identifies Adh1 and Adh4 as the enzymes sustaining fermentative ethanol production under the tested conditions. Adh4 compensates for loss of Adh1.
"only these two ADHs produce ethanol for fermentative growth"
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
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Large-scale protein localization provides the annotated mitochondrial screen observation.
"we determined the localization of 4,431 proteins"
Identification of a mitochondrial alcohol dehydrogenase in Schizosaccharomyces pombe: new insights into energy metabolism.
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Adh4-dependent mitochondrial ethanol oxidation supplies respiratory reducing equivalents; detergent latency places the active enzyme in the matrix.
"which demonstrates that the enzyme is located in the matrix"