Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
ANOVA, a putative astrocytic RNA-binding protein gene that maps to chromosome 19q13.3.
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NOVA2 contains three KH domains characteristic of RNA-binding proteins
"Screening of a human brain cDNA library with this exon identified a 1.9 kb cDNA with extensive homology to NOVA1, including three nearly identical KH domains characteristic of a subtype of RNA-binding proteins."
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NOVA2 expression is restricted to brain tissue
"Northern blots demonstrated expression of a 2.5 kb mRNA in brain, but in no other tissues."
The tetranucleotide UCAY directs the specific recognition of RNA by the Nova K-homology 3 domain.
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The Nova KH3 domain recognizes UCAY tetranucleotide motifs in RNA with sequence specificity
"we have used selection-amplification to demonstrate that the KH3 domain of Nova recognizes a single UCAY element in the context of a 20-base hairpin RNA"
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The UCAY binding motif is optimally presented as a loop element within RNA secondary structure
"the UCAY tetranucleotide is optimally presented as a loop element of the hairpin scaffold and requires protein residues C-terminal to the previously defined KH domain"
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Nova proteins regulate alternative splicing by binding intronic UCAU repeats
"The Nova-1 protein has recently been shown to regulate alternative splicing of the alpha2 glycine receptor subunit pre-mRNA by binding to an intronic element containing repeats of the tetranucleotide UCAU."
The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
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NOVA2 was identified as part of the mRNA-bound proteome in human cells
"We developed a photoreactive nucleotide-enhanced UV crosslinking and oligo(dT) purification approach to identify the mRNA-bound proteome using quantitative proteomics"
An alternative splicing program promotes adipose tissue thermogenesis.
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NOVA splicing factors suppress adipose tissue thermogenesis through alternative splicing
"Phenotypic analysis of the NOVA-deficient mice demonstrated increased adipose tissue thermogenesis and improved glycemia. We show that NOVA proteins mediate a splicing program that suppresses adipose tissue thermogenesis."
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Both NOVA1 and NOVA2 proteins are expressed in white adipocytes but not hepatocytes
"NOVA proteins were not detected in liver, but both NOVA1 and NOVA2 proteins were found in white adipocytes"
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NOVA gene expression is reduced in adipocytes of obese mice and humans
"Interestingly, Nova gene expression in white adipocytes was partially reduced in obese humans and mice"
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NOVA1 and NOVA2 show functional redundancy in adipocyte alternative splicing
"However, the effect of compound NOVA1 plus NOVA2 deficiency to cause widespread changes in alternative pre-mRNA splicing ... indicates that these NOVA proteins exhibit some functional redundancy in adipocytes."
De Novo Frameshift Variants in the Neuronal Splicing Factor NOVA2 Result in a Common C-Terminal Extension and Cause a Severe Form of Neurodevelopmental Disorder.
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NOVA2 is a major regulator of neuron-specific alternative splicing
"The neuro-oncological ventral antigen 2 (NOVA2) protein is a major factor regulating neuron-specific alternative splicing (AS)"
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De novo frameshift variants in NOVA2 cause a severe neurodevelopmental disorder (NEDASB)
"We report here six individuals with de novo frameshift variants in NOVA2 affected with a severe neurodevelopmental disorder characterized by intellectual disability (ID), motor and speech delay, autistic features, hypotonia, feeding difficulties, spasticity or ataxic gait, and abnormal brain MRI."
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NOVA2 downregulation affects splicing of 41 genes in human neural cells
"We detected 41 genes differentially spliced after NOVA2 downregulation in human neural cells."
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NOVA2 disease variants show decreased RNA binding and splicing regulatory activity
"The NOVA2 variant protein shows decreased ability to bind target RNA sequences and to regulate target AS events."
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NOVA2 is required for neurite outgrowth and axonal pathfinding
"It also fails to complement the effect on neurite outgrowth induced by NOVA2 downregulation in vitro and to rescue alterations of retinotectal axonal pathfinding induced by loss of NOVA2 ortholog in zebrafish."
Deep research report on NOVA2