Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Sequence-specific recognition of a PxLPxI/L motif by an ankyrin repeat tumbler lock.
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ANKRA2 binds megalin and class IIa HDACs.
"Ankyrin repeat family A protein 2 (ANKRA2) interacts with the plasma membrane receptor megalin and the class IIa histone deacetylases HDAC4 and HDAC5."
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ANKRA2 ankyrin repeats recognize PxLPxI/L motifs.
"We report that the ankyrin repeat domains of ANKRA2 and its close paralog regulatory factor X-associated ankyrin-containing protein (RFXANK) recognize a PxLPxI/L motif found in diverse binding proteins, including HDAC4, HDAC5, HDAC9, megalin, and regulatory factor X, 5 (RFX5)."
Characterization of ANKRA, a novel ankyrin repeat protein that interacts with the cytoplasmic domain of megalin.
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ANKRA binds the megalin cytoplasmic tail.
"Ankyrin-repeat family A protein (ANKRA) is a novel protein that interacts directly and specifically with the cytoplasmic tail of megalin in the yeast two-hybrid system and glutathione-S-transferase pull-down assays."
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ANKRA is cytosolic and on megalin-enriched membranes.
"By cell fractionation, ANKRA is found both in the cytosol and associated with membranes enriched in megalin in L2 cells and proximal tubule cells."
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ANKRA is near megalin at the plasma membrane.
"By immunofluorescence, ANKRA is concentrated near megalin along the plasma membrane of L2 cells"
Molecular mechanism of transcriptional repression of AhR repressor involving ANKRA2, HDAC4, and HDAC5.
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ANKRA2 is needed for AhRR repression.
"RNA interference experiments using ANKRA2 and AhRR siRNAs indicate that ANKRA2 is important for transcriptional repression by AhRR."
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AhRR recruits ANKRA2, HDAC4 and HDAC5 as corepressors.
"We have found that under normal conditions, CYP1A1 gene is kept silent in MEF cells by AhRR/Arnt heterodimer, which binds to the XRE sequence in its promoter and recruits ANKRA2, HDAC4, and HDAC5 as co-repressors."
Ankyrin repeats of ANKRA2 recognize a PxLPxL motif on the 3M syndrome protein CCDC8.
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CCDC8 is a major ANKRA2 partner.
"We show here that that CCDC8 is a major partner of ANKRA2 but not RFXANK in cells."
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CCDC8 links to the CUL7 ligase complex.
"The N-terminal part interacts with OBSL1 to form a CUL7 ligase complex."
Structural basis for the recognition of RFX7 by ANKRA2 and RFXANK.
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ANKRA2 recognizes the RFX7 PxLPxL motif.
"Our structural analysis reveals that both ANKRA2 and RFXANK recognize the PXLPXL motif of RFX7 and its flanking sequences via extensive hydrophobic interactions."
p53 target ANKRA2 cooperates with RFX7 to regulate tumor suppressor genes.
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ANKRA2 binds the PDCD4 X-box with RFX7.
"We identified that ANKRA2 binds to the same X-box motif in the PDCD4 promoter as RFX7"
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ANKRA2 knockdown impairs activation of RFX7 targets.
"RT-qPCR analysis showed that ANKRA2 knockdown significantly impaired the Nutlin-3a-induced activation of PDCD4 and PIK3IP1"
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RFX7 cooperates with ANKRA2 to upregulate targets.
"Based on these results, we propose a model in which RFX7 cooperates with ANKRA2 to upregulate its target genes."
Evolutionary conservation and characterization of the bare lymphocyte syndrome transcription factor RFX-B and its paralogue ANKRA2.
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ANKRA2, unlike RFX-B orthologues, cannot complement BLS MHC II deficiency.
"mouse and Xenopus RFX-B orthologues, but not the paralogous protein ANKRA2, were able to complement the MHC II deficiency in a BLS-patient-derived cell line"
Class II histone deacetylases confer signal responsiveness to the ankyrin-repeat proteins ANKRA2 and RFXANK.
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HDAC4 and HDAC5 associate with ANKRA2 and RFXANK.
"Here, we demonstrate that class II HDACs 4 and 5 physically associate with ankyrin-repeat proteins ANKRA2 and RFXANK (RFX-B/Tvl-1/ANKRA1)."
Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome.
A proteome-scale map of the human interactome network.
A reference map of the human binary protein interactome.
Proteome-scale mapping of binding sites in the unstructured regions of the human proteome.
Affinage mechanistic annotation for ANKRA2 (human)