All four seeded annotations and the original function paragraph are supported by the intact same-gene enzyme core. The selected 168 aa PC/PD sequence has 15 extra N-terminal residues, while the reviewed record questions that initiation. This discrepancy is documented without alleging a prediction-input error. PMID:1320004(https://pubmed.ncbi.nlm.nih.gov/1320004/) purified the fly enzyme and PMID:7559441(https://pubmed.ncbi.nlm.nih.gov/7559441/) connects conserved-site mutations to catalytic impairment. Native enzyme assays plus exact core conservation support the transfer.
The completed Falcon synthesis was inspected. It supports canonical NDP kinase chemistry and identifies endocytic trafficking as a well-established cellular role. The primary PMID:24528630 was cached with full text and read: live trafficking, compartment markers, mutant clones and epistasis place Awd in early-endosome maturation needed for Notch progression. This supports a NEW early-to-late-endosomal transport annotation, with ISS specifying transfer to the exact longer PC/PD product. Awd is not the receptor protease, and local GTP channeling to Rab5/dynamin is not directly established. The provider’s His119 refers to the 153-residue form; it maps to His134 in the exact accession. The extracellular-release thesis observations and disputed protein-kinase activities do not change the core NDP-kinase assessment. PMID:11768313 is a review, not a purified-protein primary experiment.