Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Automatic Gene Ontology annotation based on Rhea mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Multiple potential germ-line helicases are components of the germ-line-specific P granules of Caenorhabditis elegans.
-
Original identification of GLH-1 and GLH-2 as P granule components using polyclonal antibodies
"Two components of the germ-line-specific P granules of the nematode Caenorhabditis elgans have been identified using polyclonal antibodies specific for each"
-
GLH-1 has four CCHC zinc fingers characteristic of RNA-binding proteins
"The predicted GLH-1 protein has four CCHC fingers"
-
Both GLH proteins localize to P granules at all stages of germline development
"Both GLH proteins localize in the P granules at all stage of germ-line development"
-
Antisense RNA knockdown of glh-1 or glh-2 causes sterility in some offspring
"Injection of antisense glh-1 or glh-2 RNA into wild-type worms causes some offspring to develop into sterile adults"
The GLH proteins, Caenorhabditis elegans P granule components, associate with CSN-5 and KGB-1, proteins necessary for fertility, and with ZYX-1, a predicted cytoskeletal protein.
-
GLH-1 interacts with CSN-5 (COP9 signalosome subunit 5) by yeast two-hybrid and GST pull-down
"Three interacting proteins, CSN-5, KGB-1, and ZYX-1, were identified and further characterized. GST pull-down assays independently established that these proteins bind GLHs."
-
GLH-1 interacts with KGB-1 (JNK MAP kinase) required for fertility
"KGB-1 is a putative JNK MAP kinase that GLHs bind"
-
GLH-1 interacts with ZYX-1 (zyxin-like LIM domain protein)
"ZYX-1 is a LIM domain protein most like vertebrate Zyxin"
-
RNAi of csn-5 phenocopies glh-1/glh-4 double RNAi with sterile worms and small gonads
"RNA interference (RNAi) with csn-5 results in sterile worms with small gonads and no oocytes, a defect essentially identical to that produced by RNAi with a combination of glh-1 and glh-4"
-
Loss of CSN-5 or KGB-1 causes oogenesis to cease but does not affect initial P granule assembly
"Similar to the loss of GLH-1 and GLH-4, loss of either CSN-5 or KGB-1 causes oogenesis to cease, but does not affect the initial assembly of P granules"
GLH-1, the C. elegans P granule protein, is controlled by the JNK KGB-1 and by the COP9 subunit CSN-5.
-
GLH-1 protein levels are regulated by opposing activities of KGB-1 and CSN-5
"series of additional in vivo and in vitro tests indicates that KGB-1 and CSN-5 regulate GLH-1 levels"
-
KGB-1 promotes GLH-1 degradation by the proteasome; CSN-5 stabilizes GLH-1
"GLH-1 targeted for proteosomal degradation by KGB-1 and stabilized by CSN-5"
-
In kgb-1 mutants, GLH-1 levels are up to 6-fold elevated and P granule organization is disrupted
"GLH-1 protein levels are as much as 6-fold elevated and the organization of GLH-1 in P granules is grossly disrupted"
-
Phosphodegron motif at positions 423-427 mediates KGB-1-dependent degradation
"KGB-1 is a MAP kinase in the Jun N-terminal kinase (JNK) subfamily"
PAN-1, a P-granule component important for C. elegans fertility, has dual roles in the germline and soma.
-
PAN-1 is a novel P granule component that binds GLH-1 via GLH-1's N-terminal zinc finger region
"PAN-1, which previously has been found by others in screens for genes causing larval molting defects, is identified here as a novel P-granule component and a binding partner of GLH-1 (Germline RNA Helicase-1)"
-
PAN-1 contains leucine-rich repeats and F-box-like sequences
"The PAN-1 predicted protein contains multiple leucine-rich repeats (LRRs) and regions with similarities to F-box proteins"
-
pan-1 RNAi causes germline under-proliferation similar to glh-1 loss
"These phenocopies range from under-proliferation of the germline, as also seen with loss of GLH-1"
PGL proteins self associate and bind RNPs to mediate germ granule assembly in C. elegans.
-
GLH-1 and GLH-4 act redundantly in P granule formation in embryos
"GLH-1 and GLH-4 have partially redundant functions that are necessary for PGL proteins to form granular structures in the C. elegans adult germline"
-
glh-1 RNAi in glh-4 mutant background causes smaller P granules and abnormal PGL-3 localization
"in glh-1(RNAi) glh-4(gk225) embryos in which both GLH-1 and GLH-4 were undetectable, PGL-3 was dispersed in both somatic and germline cytoplasm"
Deep research report on GLH-1 from Falcon literature search
-
GLH-1 is a Vasa/DDX4-class ATP-dependent DEAD-box RNA helicase
-
FGG repeats at N-terminus promote perinuclear anchoring via FG-nucleoporins
-
DAAD mutations disperse GLH-1 while DQAD mutations cause dominant aggregation
-
GLH-1 coordinates recruitment of Argonaute proteins (PRG-1, CSR-1, WAGO-1) to perinuclear granules
-
ATPase cycle is essential for P granule assembly/disassembly dynamics