Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
TreeGrafter-generated GO annotations
Integrin-Mediated Adhesion in the Unicellular Holozoan Capsaspora owczarzaki.
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Integrin beta 2 localizes as patches in filopodia, with vinculin.
"We show that integrin β2 and its associated protein vinculin localize as distinct patches in the filopodia."
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An integrin beta 2 antibody inhibits adhesion to fibronectin-coated surfaces.
"Finally, using a specific antibody for integrin β2, we inhibited cell adhesion to a fibronectin-coated surface."
Integrin-mediated adhesion in the unicellular holozoan <i>Capsaspora owczarzaki</i>
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The antigen for the integrin beta 2 antibodies is CAOG_05058.
"extracellular domain of the integrin β2 (CAOG_05058, aminoacid region 733-835)"
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An antibody against the beta-I domain reduces adhesion; an antibody against the stalk does not.
"substantially reduced adhesion to fibronectin-treated surfaces, in contrast to the antibody against the"
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The natural adhesion ligand is unknown; a Capsaspora-secreted protein is proposed.
"This suggests that a protein secreted by Capsaspora sticks to the plastic substrate and provides a ligand for adhesion"
Ancient origin of the integrin-mediated adhesion and signaling machinery.
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Cation-binding sites are conserved in Capsaspora integrin betas except beta 4.
"are well conserved in the different nonmetazoan integrin β, except for C. owczarzaki integrin β4"
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The cDNAs were deposited in GenBank.
"New sequences were deposited in GenBank under accession nos. GU320672–GU320675 ."
Genome editing in the unicellular holozoan Capsaspora owczarzaki suggests a premetazoan role for the Hippo pathway in multicellular morphogenesis.