Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt.
Combined Automated Annotation using Multiple IEA Methods.
McrD binds asymmetrically to methyl-coenzyme M reductase improving active-site accessibility during assembly.
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Cryo-EM structures of M. acetivorans MCR (PDB 8GF5/8GF6) show the alpha2beta2gamma2 heterohexamer with F430, CoM and CoB in the active site and alpha Gln161 as the F430 axial ligand; McrD binds apo-MCR assembly intermediates and promotes F430 loading.
"MCR is a C2-symmetric heterohexamer defined by α2β2γ2 subunit stoichiometry"
Methanosarcina acetivorans sp. nov., an Acetotrophic Methane-Producing Bacterium Isolated from Marine Sediments.
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The type strain C2A grows and forms methane on acetate, methanol and methylamines but not on H2/CO2 or formate.
"Sodium acetate, methanol, methylamine, dimethylamine, and trimethylamine were substrates for growth and methanogenesis; H(2)-CO(2) (80:20) and sodium formate were not."
Anaerobic growth of Methanosarcina acetivorans C2A on carbon monoxide: an unusual way of life for a methanogenic archaeon.
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M. acetivorans grows on CO mainly by acetogenesis/formigenesis, with methane formation dominating only in stationary phase; it is devoid of significant hydrogen metabolism.
"Methane formation surpassed acetate/formate formation only when the cultures entered stationary growth phase"
Methyl (Alkyl)-Coenzyme M Reductases: Nickel F-430-Containing Enzymes Involved in Anaerobic Methane Formation and in Anaerobic Oxidation of Methane or of Short Chain Alkanes.
The genome of M. acetivorans reveals extensive metabolic and physiological diversity.
Identification of a unique Radical SAM methyltransferase required for the sp(3)-C-methylation of an arginine residue of methyl-coenzyme M reductase.
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The radical SAM enzyme MA4551 is required for C5-methylation of McrA Arg285; the modification is dispensable for growth but its loss impairs growth at elevated temperature and under oxidative stress without changing F430 content.
"In our study, the missing methylation of Arg285 in MCR of Mko4551 led to impaired growth at elevated temperatures and under oxidative stress, although neither the coenzyme F430 content nor the cellular MCR concentration were significantly affected by the missing methyl group."
UniProt entry Q8THH1 (MCRA_METAC)
Deep research on mcrA function