Gene Ontology annotation through association of InterPro records with GO terms
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InterPro mappings correctly identify SEP3 as a MADS-box/K-box DNA-binding transcription factor, but some propagated terms are generic and should be replaced with more specific transcription-factor and dimerization terms.
"DR InterPro; IPR002100; TF_MADSbox."
Annotation inferences using phylogenetic trees
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PANTHER/IBA placement in the MADS-box/MEF2 transcription-factor family is consistent with SEP3's conserved DNA-binding transcription-factor role.
"O22456,Developmental protein SEPALLATA 3,protein,3702,Arabidopsis thaliana,Arabidopsis thaliana (Mouse-ear cress),SEP3,251"
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Local UniProt record for Arabidopsis SEP3 (O22456)
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UniProt describes SEP3 as a probable transcription factor in inflorescence development and floral organogenesis.
"Probable transcription factor active in inflorescence development and floral organogenesis."
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UniProt records SEP3 nuclear localization.
"SUBCELLULAR LOCATION: Nucleus"
Local GOA annotations for Arabidopsis SEP3
Falcon deep research report for Arabidopsis SEP3
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Deep research synthesis identifies SEP3's core roles as DNA-binding transcription-factor activity, MADS complex formation, floral organ identity and floral meristem determinacy.
"SEP3 is a central E-class cofactor required, redundantly with SEP1/SEP2/SEP4, for floral organ identity and floral meristem determinacy."
PANTHER family PTHR48019 metadata
PANTHER family PTHR48019 member entries
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SEP3 is a member of PTHR48019.
"O22456,Developmental protein SEPALLATA 3,protein,3702,Arabidopsis thaliana,Arabidopsis thaliana (Mouse-ear cress),SEP3,251"
B and C floral organ identity functions require SEPALLATA MADS-box genes.
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SEP1/2/3 are required for petal, stamen and carpel identity.
"SEP1/2/3 are a class of organ-identity genes that is required for development of petals, stamens and carpels."
Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
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Arabidopsis has a large transcription-factor repertoire; this is family-level support rather than SEP3-specific functional evidence.
"Arabidopsis dedicates over 5% of its genome to code for more than 1500 transcription factors"
APETALA1 and SEPALLATA3 interact to promote flower development.
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SEP3 interacts with AP1 to promote flower development.
"Taken together, these studies suggest that SEP3 interacts with AP1 to promote normal flower development."
Molecular and phylogenetic analyses of the complete MADS-box transcription factor family in Arabidopsis: new openings to the MADS world.
MADS-box protein complexes control carpel and ovule development in Arabidopsis.
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SEP proteins are required for MADS complexes that control normal ovule development.
"a reduction in SEP activity leads to the loss of normal ovule development"
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AG/SHP/STK MADS complex formation requires SEP proteins.
"STK, AG, SHP1, and SHP2 can form multimeric complexes and that these interactions require the SEPALLATA (SEP) MADS-box proteins."
Analysis of the Arabidopsis nuclear proteome and its response to cold stress.
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This high-throughput nuclear-proteome source supports a nuclear context, but the cached abstract does not identify SEP3 specifically.
"In this study, we comprehensively characterized the Arabidopsis nuclear proteome."
Defining subdomains of the K domain important for protein-protein interactions of plant MADS proteins.
Comprehensive interaction map of the Arabidopsis MADS Box transcription factors.
Mutant analysis, protein-protein interactions and subcellular localization of the Arabidopsis B sister (ABS) protein.
APETALA1 and SEPALLATA3 interact with SEUSS to mediate transcription repression during flower development.
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SEP3 binds the SEUSS transcriptional corepressor.
"APETALA1 (AP1) and SEPALLATA3 (SEP3), both MADS box DNA-binding proteins, interacted with SEU."
A Bsister MADS-box gene involved in ovule and seed development in petunia and Arabidopsis.
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B-sister/C/E and B-sister/D/E MADS proteins form higher-order complexes in ovule and seed development.
"Protein interaction studies revealed the formation of higher-order complexes between B(s)-C-E and B(s)-D-E type MADS-box proteins"
The class E floral homeotic protein SEPALLATA3 is sufficient to loop DNA in 'floral quartet'-like complexes in vitro.
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SEP3 binds cooperatively to CArG-box DNA as a homotetramer.
"homotetramers of the class E floral homeotic protein SEPALLATA3 from Arabidopsis thaliana bind cooperatively to two sequence elements termed 'CArG boxes'"
Target genes of the MADS transcription factor SEPALLATA3: integration of developmental and hormonal pathways in the Arabidopsis flower.
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SEP3 binds thousands of genomic sites in floral tissues.
"SEPALLATA3 binds to thousands of sites in the genome."
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SEP3 is a key component of the floral organ regulatory transcriptional network.
"this protein is a key component in the regulatory transcriptional network underlying the formation of floral organs."
Characterization of MADS-domain transcription factor complexes in Arabidopsis flower development.
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AP1, AP3, PI, AG and SEP3 interact in floral tissues in a manner consistent with the floral quartet model.
"five major floral homeotic MADS-domain proteins (AP1, AP3, PI, AG, and SEP3) interact in floral tissues as proposed in the "floral quartet" model."
Tetramerization of MADS family transcription factors SEPALLATA3 and AGAMOUS is required for floral meristem determinacy in Arabidopsis.
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SEP3/AG tetramerization is required for floral meristem determinacy and activation of determinacy targets including KNUCKLES and CRABSCLAW.
"The heterotetramer formed by SEP3 and the MADS protein, AGAMOUS, is necessary to activate two target genes, KNUCKLES and CRABSCLAW, which are required for meristem determinacy."
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Tetramerization-defective SEP3 complementation separates organ identity restoration from floral meristem determinacy.
"In contrast, while the carpel development program was launched, gynoecium development is incomplete due to a lack of determinacy of the floral meristem."
SWI2/SNF2 chromatin remodeling ATPases overcome polycomb repression and control floral organ identity with the LEAFY and SEPALLATA3 transcription factors.
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SEP3 physically interacts with SWI/SNF chromatin remodelers.
"physically interact with two direct transcriptional activators of class B and class C gene expression, LEAFY (LFY) and SEPALLATA3 (SEP3)."
Phytoplasma effector SAP54 hijacks plant reproduction by degrading MADS-box proteins and promotes insect colonization in a RAD23-dependent manner.
Identification of novel seed longevity genes related to oxidative stress and seed coat by genome-wide association studies and reverse genetics.
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SEP3 is associated with seed longevity and a protective seed coat role, but specific seed coat and mucilage GO annotations cannot be checked from the cached abstract alone.
"The cytochrome P-450 hydroxylase, CYP86A8, and the transcription factors, MYB47, KNAT7 and SEP3, support the protecting role of the seed coat during seed ageing."
The protein-protein interaction landscape of transcription factors during gynoecium development in Arabidopsis.
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The abstract supports a large gynoecium transcription-factor interaction map, but exact SEP3 partner evidence cannot be checked locally.
"We analyzed almost 4500 combinations and detected more than 250 protein-protein interactions"
Protein interaction mapping reveals widespread targeting of development-related host transcription factors by phytoplasma effectors.
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Phytoplasma effector interaction evidence belongs to host-pathogen targeting context, not SEP3's endogenous molecular function.
"widespread, but specific, interactions between phytoplasma effectors and host transcription factors"