Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Ama1p is a meiosis-specific regulator of the anaphase promoting complex/cyclosome in yeast.
Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes.
Mnd2 and Swm1 are core subunits of the Saccharomyces cerevisiae anaphase-promoting complex.
An architectural map of the anaphase-promoting complex.
The anaphase-promoting complex promotes actomyosin-ring disassembly during cytokinesis in yeast.
The social and structural architecture of the yeast protein interactome.
Budding yeast RSI1/APC2, a novel gene necessary for initiation of anaphase, encodes an APC subunit.
Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins.
The APC11 RING-H2 finger mediates E2-dependent ubiquitination.
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Apc11 alone mediates E2-dependent ubiquitin transfer in vitro; Apc2 was not required in the minimal system and is proposed to tether and position Apc11 within the complex.
"While Apc2p was not required to effect ubiquitin transfer in vitro, it may function in a cellular context to tether Apc11p to APC core proteins, and to properly position the Apc11p RING-H2 finger with respect to its substrates."
Identification of a physiological E2 module for the human anaphase-promoting complex.
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The yeast APC/C uses Ubc4 to initiate and Ubc1 to elongate K48-linked chains, in contrast to the K11-linked chains built by human UBE2C/UBE2S.
"However, Ubc4 and Ubc1 function sequentially to assemble K48-linked ubiquitin chains, whereas human UbcH10 and Ube2S most likely bind APC/C at the same time."
UniProtKB record for S. cerevisiae APC2 (Q12440)
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Apc2 anchors Apc11 to the APC/C core complex and belongs to the cullin family.
"APC2 interacts directly with APC11 thereby anchoring APC11 to the core complex."
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GFP localisation places Apc2 in cytoplasm and nucleus.
"SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus {ECO:0000269|PubMed:14562095}."
Deep research summary for yeast APC2 (Edison/falcon)
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Localisation evidence for the yeast APC/C rests on tagged Cdc23 and Apc9, which are predominantly nuclear; Apc2 itself was not imaged in those studies.
"Because these studies visualized Cdc23 or Apc9 rather than Apc2, this is complex-level localization evidence and should not be overinterpreted as a directly measured Apc2 distribution."
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Apc2 acts architecturally, organising Apc11 and the E2-binding geometry.
"Apc2's contribution is architectural: it organizes Apc11 and the E2-binding geometry rather than independently catalyzing a small-molecule reaction."