Tes (rat, Q2LAP6) review notes

• 2026-10-28: just fetch-gene rat Tes resolved Q2LAP6 (TES_RAT, RGD:1566346, GeneID 500040); 13 GOA rows,
  all IBA/IEA/ISS/ISO (no rat experimental rows). No deep research run (no API keys); see
  Tes-deep-research-manual.md for the literature synthesis.
• Key rat paper PMID:21278383 (full text): Tes-Arp7A-Mena complex co-IP from rat testis, and localisation
  to the subacrosomal layer (acroplaxome) of round spermatids PMID:21278383.
  GO has no acroplaxome CC term (searched acrosom in GO); not proposed as NEW.
• Decisions:
• focal adhesion (IEA, ISO): ACCEPT, core location [PMID:12571287; PMID:12695497].
• cytoplasm (IBA, IEA, ISS, ISO) and cytosol (ISO): KEEP_AS_NON_CORE; diffuse pool, protein shuttles among
  cytosol, stress fibres and FAs.
• zinc ion binding (IEA, ISS, ISO): KEEP_AS_NON_CORE; structural Zn in LIM domains PMID:21278383.
• negative regulation of cell population proliferation (ISS, ISO): KEEP_AS_NON_CORE; overexpression growth
  suppression in tumour lines [PMID:11420696; PMID:12695497].
• protein-containing complex (ISO): KEEP_AS_NON_CORE; rat co-IP supports it directly, no more specific GO
  complex term exists for Arp7A-Tes-Mena.
• NEW: cytoskeletal protein binding (GO:0008092, IPI PMID:21278383) as the informative MF for the
  LIM-domain scaffold (Mena EVH1, zyxin, Arp7A). No NEW BP proposed (spreading/migration/stress fibre
  data are overexpression/knockdown phenotypes in non-rat cells).