Coordinate regulation of bacterial virulence genes by a novel adenylate cyclase-dependent signaling pathway.
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A membrane-associated adenylate cyclase originates a signalling pathway controlling TTSS gene expression.
"led to the identification of a signaling pathway that originates from a membrane-associated adenylate cyclase and controls TTSS gene expression"
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Mutants unable to synthesise cAMP or lacking Vfr were profiled.
"Transcriptome analysis of mutants lacking the ability to synthesize cAMP or the cAMP binding protein Vfr"
Crystal structure and regulation mechanisms of the CyaB adenylyl cyclase from the human pathogen Pseudomonas aeruginosa.
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Purified CyaB catalytic domain has adenylyl cyclase activity stimulated by CO2/bicarbonate.
"CO2/HCO3− stimulated CyaB217–463 activity in the presence of either divalent, with optimal stimulation occurring at pH 7."
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CyaB makes most intracellular cAMP.
"CyaB activity indeed accounts for the majority of intracellular cAMP"
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Full-length CyaB fractionates with the inner membrane; the MASE2 domain is the anchor.
"Full-length CyaB and CyaBΔ1–17 were detected exclusively in the total membrane and inner membrane fractions"
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Electronic Gene Ontology annotations created by ARBA machine learning models