Gene Ontology annotation through association of InterPro records with GO terms

Annotation inferences using phylogenetic trees

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:10944123

Initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-P and is regulated by DPM2.

GPI-GnT requires PIG-P as an essential component and is regulated by DPM2, which associates with the complex through interactions with PIG-A, PIG-C and GPI1 and enhances enzyme activity ~3-fold.

PMID:16162815

The initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-Y, a seventh component.

PIGA is the catalytic subunit of the GPI-GnT complex; PIG-Y is directly associated with PIG-A and regulates GPI-GnT activity. This study established the catalytic activity and seven-component complex (EC 2.4.1.198).

PMID:19946888

Defining the membrane proteome of NK cells.

High-throughput membrane proteome of the NK-like cell line YTS; supports only a generic membrane localization for PIGA.

PMID:33961781

Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.

BioPlex 3.0 AP-MS interactome; recovers PIGA interactions with GPI-GnT complex members (PIGP, PIGH), supporting a generic protein-binding annotation.

PMID:8900170

PIG-A and PIG-H, which participate in glycosylphosphatidylinositol anchor biosynthesis, form a protein complex in the endoplasmic reticulum.

PIG-A is an ER transmembrane protein with a large cytoplasmic (catalytic) domain and a small lumenal domain that targets it to the rough ER; PIG-A and PIG-H form a complex and transfer GlcNAc to PI on the cytoplasmic side of the ER.

PMID:9463366

The first step of glycosylphosphatidylinositol biosynthesis is mediated by a complex of PIG-A, PIG-H, PIG-C and GPI1.

The first step of GPI biosynthesis (GlcNAc transfer from UDP-GlcNAc to PI) is mediated by an ER-membrane complex of PIG-A, PIG-H, PIG-C and GPI1 that has GPI-GnT activity in vitro.

Reactome:R-HSA-162710

Synthesis of glycosylphosphatidylinositol (GPI)

Reactome:R-HSA-162730

phosphatidylinositol + UDP-N-acetyl-D-glucosamine -> N-acetylglucosaminyl-PI + UDP

file:human/PIGA/PIGA-uniprot.txt

UniProtKB P37287 (PIGA_HUMAN) record

Catalytic subunit of the GPI-GnT complex that catalyzes transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol (EC 2.4.1.198); rough ER membrane, single-pass membrane protein; complex composed of PIGA, PIGC, PIGH, PIGP, PIGQ, PIGY and DPM2.