Human reference: https://www.uniprot.org/uniprotkb/Q14156/entry
Primary source: PMID:23229899 PtdIns4P synthesis by PI4KIIIα at the plasma membrane and its impact on plasma membrane identity..
Accordingly, a bioorthogonal metabolic labeling approach (Hang et al., 2011) revealed that FLAG-tagged EFR3A and EFR3B were palmitoylated at the N-terminal Cys-rich motif (Fig. 4 B). Importantly, palmitoylation-deficient mutants (C6S/C7S/C8S/C9S for EFR3A and C5S/C7S/C8S for EFR3B) were localized in the cytosol (Fig. 4 C), and the EFR3B palmitoylation-deficient mutant was unable to recruit tagged TTC7B and M1-PI4KIIIα to the plasma membrane (Fig. S2).
The horse sequence comparison is in EFR3A-bioinformatics/RESULTS.md. Transfer is assessed for this exact accession; human biochemical evidence is not equine experimental validation.