Gene Ontology annotation through association of InterPro records with GO terms
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
UniProtKB record A0A0H2M677 (A0A0H2M677_VARPD) - Putative TonB-dependent receptor BfrD from Variovorax paradoxus TBEA6
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BfrD1 (VPARA_25830) is a 739-aa outer-membrane TonB-dependent siderophore receptor containing a signal peptide (residues 1-17), an N-terminal plug domain (Pfam PF07715, residues 60-159), and a C-terminal beta-barrel domain (Pfam PF00593, residues 265-709).
"SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000256|ARBA:ARBA00004571, ECO:0000256|PROSITE-ProRule:PRU01360}; Multi-pass membrane protein"
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VPARA_25830 corresponds to EMBL CDS accession KLN56277.1 on genomic contig JZWI01000012, where consecutive CDS accessions (KLN56276.1-KLN56277.1) and locus tags stepping by 10 place bfrD1 (VPARA_25830) immediately downstream of tonB (VPARA_25820, KLN56276.1).
"DR EMBL; JZWI01000012; KLN56277.1; -; Genomic_DNA."
TonB-dependent transporters: regulation, structure, and function.
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Gram-negative outer-membrane TonB-dependent transporters consist of a 22-stranded beta-barrel occluded by an N-terminal plug domain that binds ferric-siderophore chelates on the extracellular side and interacts with the TonB-ExbB-ExbD complex on the periplasmic side.
"The first crystal structures of two Escherichia coli TonB-dependent transporters, ferrichrome transporter (FhuA) (34, 54) and ferric enterobactin transporter (FepA) (10), showed that TBDTs use a 22-stranded β-barrel to span the outer membrane with an unanticipated ‘plug’ domain folded into the barrel interior. The plug domain functions to bind a specific metal chelate at the extracellular side of the membrane and to interact with TonB-ExbB-ExbD at the periplasmic side of the outer membrane."
An automated Genomes-to-Natural Products platform (GNP) for the discovery of modular natural products.
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Isolation from Variovorax paradoxus P4B extracts yielded the novel siderophore natural products variobactin A and B.
"The crude extract of V. paradoxus P4B was shown to contain a series of candidate hits (Fig. 2b) whose isolation yielded the novel natural products variobactin A and B"