UniProt gene name "spoOJ" (with a letter O) is the PAO1 submitter name; the literature uses parB.
Experimental evidence.
- Purified ParB binds parS; C-terminal 56 aa dimerization domain; ParA interaction; parS-dependent
silencing of nearby genes on overproduction PMID:15466051.
- parB deletions: anucleate cells, growth and motility defects, irregular foci PMID:19332810.
- Spreading-deficient but DNA-binding-competent alleles phenocopy the null; source of the IPI
self-interaction annotation PMID:21531806.
- C-terminal leucine-zipper-like residues L282, V285, I289 are needed for dimerization and every
function PMID:22322962.
- Ten parS sites; any one of parS1-4 is necessary and sufficient PMID:25794281. ParB binds
parS1-4 in vivo, and parS is the first locus separated after replication PMID:27820816.
- ParB is a nucleoid-associated protein binding hundreds of half-parS sites PMID:29648658, with
occupancy shifting to half-parS on entry into stationary phase PMID:37569892.
- Direct repression of genes adjacent to parS3/parS4 PMID:28732084.
- The N-terminal motif of ParB binds ParA; other partners include NdpA, MinE, CTP synthetase and
FleN [PMID:36622167, abstract only].
- CTPase: purified ParB hydrolyses CTP with KM 44 uM, stimulated fully by parS and partly by
non-specific DNA; a sterigmatocystin derivative inhibits it PMID:38843873.
- ParB is synthetically lethal with the condensins SMC and MksB PMID:32907947.
GOA rows. DNA binding → MODIFY to sequence-specific DNA binding. Chromosome and chromosome
segregation → ACCEPT. Identical protein binding → MODIFY to protein homodimerization activity.
Regulation of transcription → MODIFY to negative regulation (non-core). DNA-templated
transcription → REMOVE. Positive regulation of sporulation (IBA, B. subtilis donor) → REMOVE.
NEW: CTPase activity (IDA), sequence-specific DNA binding (IDA).
The transcription rows come from UniRule UR000413841. This entry, unlike the P. putida
ortholog, has a PROSITE HTH_CROC1 (PS50943) / IPR001387 match, which may explain why only the
P. aeruginosa protein gets transcription terms. This is an inference: the conditions of
UR000413841 were not checked.