Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
AGPAT6 is a novel microsomal glycerol-3-phosphate acyltransferase.
The microsomal cardiolipin remodeling enzyme acyl-CoA lysocardiolipin acyltransferase is an acyltransferase of multiple anionic lysophospholipids.
A reference map of the human binary protein interactome.
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
G3P is acylated to 1-acyl LPA by GPAM/GPAT2 (OM)
Expression of Glycerol-3-phosphate Acyltransferase (GPAM, GPAT)
Triglyceride biosynthesis
glycerol 3-phosphate + acyl-CoA => 1-acylglycerol 3-phosphate + CoASH [mitochondrial membrane-associated]
GPAM gene expression is stimulated by RUNX1 and ESR1
Structural basis of the acyl-transfer mechanism of human GPAT1.
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Cryo-EM structures of human GPAT1 show an N-terminal acyltransferase domain with the catalytic motifs, a C-terminal domain needed for folding, and peripheral membrane association via an amphipathic patch and an N-terminal loop-helix containing the mitochondrial-targeting signal (no transmembrane helices).
"Unexpectedly, GPAT1 has no transmembrane regions as previously proposed but instead associates with the membrane via an amphipathic surface patch and an N-terminal loop-helix region that contains a mitochondrial-targeting signal."
Mitochondrial glycerol-3-phosphate acyltransferase-deficient mice have reduced weight and liver triacylglycerol content and altered glycerolipid fatty acid composition.
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Gpam-null mice have lower hepatic and plasma triacylglycerol and less palmitate at sn-1 of hepatic PC and PE, establishing an in vivo role of the SF16 ortholog in triacylglycerol synthesis and phospholipid acyl composition.
"These data confirm the important role of mtGPAT in the synthesis of triacylglycerol"
Glycerol-3-phosphate acyltransferase (GPAT)-1, but not GPAT4, incorporates newly synthesized fatty acids into triacylglycerol and diminishes fatty acid oxidation.
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In mouse hepatocytes GPAT1, but not GPAT4, routes de novo synthesised fatty acids into TAG and away from oxidation.
"GPAT1, but not GPAT4, is required to incorporate de novo synthesized fatty acids into TAG and to divert them away from oxidation."
Regulation of Triglyceride Metabolism. II. Function of mitochondrial GPAT1 in the regulation of triacylglycerol biosynthesis and insulin action.
Glycerophosphate/Acylglycerophosphate acyltransferases.
UniProtKB Q9HCL2 (GPAT1_HUMAN) record