FlyBase identifies one 525 aa TyrRS-PA/Q9VV60 protein. The catalogue PMID:26761199(https://pubmed.ncbi.nlm.nih.gov/26761199/) distinguishes cytoplasmic TyrRS/CG4561 from mitochondrial TyrRS-m. PMID:19561293(https://pubmed.ncbi.nlm.nih.gov/19561293/) provides functional and genetic complementation support, though this cache is abstract-only and no new construct-specific assay claim is made.
The charging narrative is supported. The resveratrol claim remains UNC. The starvation prediction is CNN on direct fly secretion evidence, scoped to a cellular response to serum deprivation. Direct binding is not refuted by the obsoletion of a nonevolved ligand-binding GO term. QuickGO snapshots verify both resveratrol-term obsoletion and replacement guidance for the obsolete tyrosyl-tRNA aminoacylation BP term. Original IDs/labels remain untouched.
Falcon completed after its wrapper timed out. A separate manual research synthesis documents directly inspected evidence. The completed Falcon report was read and its endogenous secretion finding is evaluated against the primary paper. The human resveratrol/serum-starvation study PMID:25533949 was fetched with full text and read; its direct evidence is acknowledged while transfer to native fly TyrRS remains unresolved.
The full PMID:26658841 article was downloaded and its Results, figures and controls read. The immutable HTML and extracted text are retained locally. Endogenous extracellular staining and haemolymph blots support secretion during competition; TyrRS depletion reduces haemocyte recruitment, while secreted TyrRS/EMAP constructs support chemoattractant activity. Figure 3 reports TyrRS-HA secretion after 24 h serum deprivation in S2 cells with anti-tubulin and membrane-integrity controls. This supports the broad starvation-response prediction under the QuickGO definition, which explicitly includes secretion changes upon nourishment deprivation. It does not establish whole-animal starvation fitness or the rat donor’s nuclear PARP1 mechanism. The cached PMID record is abstract-only, but the separately retained publisher full text supplies the Figure 3 evidence.