Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Combined Automated Annotation using Multiple IEA Methods
Transcriptional comparison of the filamentous fungus Neurospora crassa growing on three major monosaccharides D-glucose, D-xylose and L-arabinose.
Identification and characterization of the glucose dual-affinity transport system in Neurospora crassa: pleiotropic roles in nutrient transport, signaling, and carbon catabolite repression.
Molecular determinants for the thermodynamic and functional divergence of uniporter GLUT1 and proton symporter XylE.
-
XylE and GLUT1 illustrate sugar-porter symporter versus uniporter divergence, with the TM1 XylE Asp27 / GLUT1 Asn29 position contributing to the mechanistic split.
"studies of XylE and GLUT1 suggest that the variation between an acidic residue (Asp27 in XylE) and a neutral one (Asn29 in GLUT1) is a key element for their mechanistic divergence"
Crystal structure of the human glucose transporter GLUT1.
-
The same sugar-porter fold includes the passive uniporter GLUT1 and proton-coupled XylE, so mechanism is not conserved across GLUT1/XylE homologs.
"Structural comparison of the uniporter GLUT1 with its bacterial homologue XylE, a proton-coupled xylose symporter, allows examination of the transport mechanisms of both passive facilitators and active transporters."
Kinetic characteristics of the two glucose transport systems in Neurospora crassa.
-
Historical whole-cell measurements distinguished a constitutive low-affinity Neurospora glucose system I from high-affinity glucose system II.
"The apparent K(m) for glucose uptake by system I and system II are 25 and 0.04 mm, respectively."
Characterization and regulation of galactose transport in Neurospora crassa.
-
Carbon starvation induces an energy-requiring high-affinity general monosaccharide uptake activity corresponding to glucose transport system II.
"In carbon-starved mycelia or mycelia incubated with galactose, a second galactose transport activity appeared which required energy, had a high affinity for galactose (Km = 0.7 mM), and was shown to be the same as glucose transport system II."
Identification of the mstE gene encoding a glucose-inducible, low affinity glucose transporter in Aspergillus nidulans.
-
MstE, the A. nidulans member of the PANTHER subfamily that contains GLT-1, is a low-affinity glucose transporter located at the cell periphery consistent with the plasma membrane.
"fluorescence at the periphery of conidia and hyphae is consistent with MstE location in the plasma membrane"
GLT-1 TM1 protonation-site re-check (2026-10-21 re-review)
-
GLT-1 Val48 and SF92 relative MstE Ser67 occupy the XylE Asp27 TM1 protonation site, whereas Neurospora HGT-1 and HGT-2 retain Asp.
"GLT-1 has a non-protonatable Val48 at this site, and the characterised low-affinity glucose transporter MstE from the same PANTHER subfamily (SF92) has a non-acidic Ser"
OpenScientist focused report on glt-1 glucose transport and proton coupling
-
The focused report found that the live PANTHER ancestry for GO:0005351 is valid but does not establish GLT-1 proton coupling.
"procedural ancestry validity ≠ demonstrated mechanism"
-
The focused report found that GLT-1 lacks the TM1 acidic residue retained by high-affinity Neurospora HGT-1 and HGT-2 proton-coupled paralogs.
"GLT-1 has **Val48** at the XylE-Asp27 position"