ATG14 PN Consistency Notes
- Generated: 2026-06-18
- Project: PROTEOSTASIS
- Scope: PN consistency rereview against local AIGR review and available deep-research artifacts
- UniProt: Q6ZNE5
- AIGR review status: COMPLETE
- Review batch: proteostasis-pr-1217 (PR 1217)
- Batch change status: added
Source Files Checked
Deep Research Files
AIGR Review Snapshot
- Description: ATG14/Barkor/ATG14L is the autophagy-specific regulatory and membrane-adaptor subunit of the human class III PI3K complex I (PI3KC3-C1). It targets the PIK3C3/VPS34-PIK3R4/VPS15-BECN1 complex to ER-associated omegasome/phagophore membranes for local PI3P production and autophagosome assembly, and it also promotes later STX17-SNAP29/VAMP8-dependent autophagosome-endolysosome fusion. Its main cellular roles are autophagy initiation and autophagosome maturation rather than class I PI3K/AKT signaling.
- Existing/core annotation action counts: ACCEPT: 45; KEEP_AS_NON_CORE: 11; MARK_AS_OVER_ANNOTATED: 24; MODIFY: 12; REMOVE: 3
PN Consistency Summary
- Consistency: Strongly consistent. The PN dual placement (early PI3KC3-C1 component + late STX17-SNAP29/VAMP8 fusion regulator) is exactly the two-function picture the review builds: PI3KC3-C1 recruitment/PI3P production AND ATG14-promoted autophagosome-endolysosome tethering/fusion (GO:0016240, GO:0097352 accepted; PMID:25686604). The projected GO:0034271 is precisely the review's MODIFY target — every GO:0035032 row is MODIFY→GO:0034271. No contradiction.
- PN story / NEW pressure: Already captured. The projected GO:0034271 is the review's own replacement term (not broader — it is narrower than existing GOA, the good direction). The late-fusion SNARE-regulator placement is already covered by accepted GO:0016240/GO:0097352. No new term warranted; PN adds no role the review lacks.
- Evidence alignment: Partial overlap. PN cites the Nature paper "ATG14 promotes membrane tethering and fusion of autophagosomes to endolysosomes" = review's PMID:25686604 (heavily used). PN's two review-article citations (Annual Review; tandfonline) are not in the review, which instead uses primary literature (PMID:20713597, 21518905, 40442316, etc.). Convergent on the load-bearing paper.
- Verdict: Fully consistent; PN projection matches the review's own MODIFY. No edits needed.
Full Consistency Review
- UniProt: Q6ZNE5 · batch: proteostasis-pr-1217 · review status: COMPLETE
- PN placement: 2 rows, ALP — (1)
Autophagophore initiation and elongation → Class 3 PI3K complex 1, direct → Class 3 PI3K complex 1 component; (2) Autophagosome closure maturation and lysosome fusion → Autophagosome-lysosome docking → Lysosome-autophagosome SNARE complex regulator. PN-node mapping: component leaf=mapped→GO:0034271 (PI3KC3 type I); SNARE-regulator leaf=no_mapping; ancestors context_only (GO:0035032, GO:0061909, GO:0016236). Projected: GO:0034271 (more_specific_than_existing_goa).
- Consistency: Strongly consistent. The PN dual placement (early PI3KC3-C1 component + late STX17-SNAP29/VAMP8 fusion regulator) is exactly the two-function picture the review builds: PI3KC3-C1 recruitment/PI3P production AND ATG14-promoted autophagosome-endolysosome tethering/fusion (GO:0016240, GO:0097352 accepted; PMID:25686604). The projected GO:0034271 is precisely the review's MODIFY target — every GO:0035032 row is MODIFY→GO:0034271. No contradiction.
- PN story / NEW pressure: Already captured. The projected GO:0034271 is the review's own replacement term (not broader — it is narrower than existing GOA, the good direction). The late-fusion SNARE-regulator placement is already covered by accepted GO:0016240/GO:0097352. No new term warranted; PN adds no role the review lacks.
- Mapping strategy: No change needed. ATG14 strengthens the GO:0034271 mapping (it is the defining C1 subunit). The SNARE-regulator leaf's no_mapping is correct — ATG14's fusion role is already individually annotated, so leaf-level propagation would be redundant. This is the inverse of the TOMM20/HSPA8 over-reach pattern: here the projection is narrower than GOA, and the review agrees.
- Evidence alignment: Partial overlap. PN cites the Nature paper "ATG14 promotes membrane tethering and fusion of autophagosomes to endolysosomes" = review's PMID:25686604 (heavily used). PN's two review-article citations (Annual Review; tandfonline) are not in the review, which instead uses primary literature (PMID:20713597, 21518905, 40442316, etc.). Convergent on the load-bearing paper.
- Verdict: Fully consistent; PN projection matches the review's own MODIFY. No edits needed.
PN Dossier Context
- review_batch: proteostasis-pr-1217
- review_yaml: genes/human/ATG14/ATG14-ai-review.yaml
- PN workbook rows: 2
PN row 1: Autophagy-Lysosome Pathway | Autophagophore initiation and elongation | Class 3 PI3K complex 1, direct | Class 3 PI3K complex 1 component
- UniProt: Q6ZNE5
- In branches: ALP
- Notes: Member of class III PI3K complex 1 that produces PI(3)P at the site of phagophore nucleation. Targets the complex to the ER membrane. Also homooligomerizes and binds to STX17 in the STX17-SNAP29-VAMP8 SNARE complex to regulate autophagosome-lysosome fusion.
- PN references (titles):
- Mammalian Autophagy: How Does It Work? | Annual Review of Biochemistry (annualreviews.org)
- Full article: Autophagy pathway: Cellular and molecular mechanisms (tandfonline.com)
- ATG14 promotes membrane tethering and fusion of autophagosomes to endolysosomes | Nature
- PN-node mapping records (path + ancestors):
- [type] Autophagy-Lysosome Pathway|Autophagophore initiation and elongation|Class 3 PI3K complex 1, direct|Class 3 PI3K complex 1 component
status=mapped scope=ok_for_propagation_to_go GO=[GO:0034271 phosphatidylinositol 3-kinase complex, class III, type I]
rationale: This PN type is a curated component class for the direct autophagy- promoting class III PI3K complex 1. Propagation to the matching GO cellular-component term is appropriate, although the source is a component-role category rather than the complex term itself.
- [group] Autophagy-Lysosome Pathway|Autophagophore initiation and elongation|Class 3 PI3K complex 1, direct
status=context_only scope=too_broad_to_propagate GO=[GO:0035032 phosphatidylinositol 3-kinase complex, class III]
rationale: Reviewed as a class-III PI3K complex context or regulator bucket. This node is useful for curator interpretation, but it should not project cellular-component membership; only explicit complex-component leaves propagate to GO complex terms.
- [class] Autophagy-Lysosome Pathway|Autophagophore initiation and elongation
status=context_only scope=too_broad_to_propagate GO=[GO:0016236 macroautophagy]
rationale: This class is a real macroautophagy context, but its descendants include core factors, component buckets, upstream modulators, localization roles, and residual categories. Projecting generic macroautophagy from this ancestor creates TRAPP-like overpropagation, so candidate GO annotations must come from narrower curated nodes.
- [branch] Autophagy-Lysosome Pathway
status=no_mapping scope= GO=[]
rationale: Reviewed as the top-level PN branch. It is a project taxonomy umbrella rather than a direct GO assertion; all propagation must come from manually curated child nodes.
PN row 2: Autophagy-Lysosome Pathway | Autophagosome closure maturation and lysosome fusion | Autophagosome-lysosome docking | Lysosome-autophagosome SNARE complex regulator
- UniProt: Q6ZNE5
- In branches: ALP
- Notes: Member of class III PI3K complex 1 that produces PI(3)P at the site of phagophore nucleation. Targets the complex to the ER membrane. Also homooligomerizes and binds to STX17 in the STX17-SNAP29-VAMP8 SNARE complex to regulate autophagosome-lysosome fusion.
- PN references (titles):
- Mammalian Autophagy: How Does It Work? | Annual Review of Biochemistry (annualreviews.org)
- Full article: Autophagy pathway: Cellular and molecular mechanisms (tandfonline.com)
- ATG14 promotes membrane tethering and fusion of autophagosomes to endolysosomes | Nature
- PN-node mapping records (path + ancestors):
- [type] Autophagy-Lysosome Pathway|Autophagosome closure maturation and lysosome fusion|Autophagosome-lysosome docking|Lysosome-autophagosome SNARE complex regulator
status=no_mapping scope= GO=[]
rationale: This PN leaf groups regulators placed around the lysosome-autophagosome SNARE complex, but the current member set mixes broad trafficking, ubiquitin, and autophagy-fusion factors rather than one clean shared GO term for SNARE-complex regulation.
- [group] Autophagy-Lysosome Pathway|Autophagosome closure maturation and lysosome fusion|Autophagosome-lysosome docking
status=context_only scope=too_broad_to_propagate GO=[GO:0061909 autophagosome-lysosome fusion]
rationale: Reviewed as an autophagosome-lysosome docking context. The subtree mixes component buckets and modulators, so generic fusion propagation should come only from narrower reviewed mechanism leaves.
- [class] Autophagy-Lysosome Pathway|Autophagosome closure maturation and lysosome fusion
status=context_only scope=too_broad_to_propagate GO=[GO:0016236 macroautophagy]
rationale: This class is a late macroautophagy context, but the subtree mixes docking, fusion, localization, membrane-composition, and unknown late-stage roles. The class-level relation is useful for display while propagation is restricted to narrower mechanism nodes.
- [branch] Autophagy-Lysosome Pathway
status=no_mapping scope= GO=[]
rationale: Reviewed as the top-level PN branch. It is a project taxonomy umbrella rather than a direct GO assertion; all propagation must come from manually curated child nodes.
Projected GO annotations (1)
- GO:0034271 phosphatidylinositol 3-kinase complex, class III, type I | scope=ok_for_propagation_to_go | goa_status=more_specific_than_existing_goa | from=Autophagy-Lysosome Pathway|Autophagophore initiation and elongation|Class 3 PI3K complex 1, direct|Class 3 PI3K complex 1 component
Note
This file is generated from the current PROTEOSTASIS phase-1 dossier and local gene-review artifacts. Edit the source review, PN mapping, or dossier rather than this generated note when correcting the underlying curation.