GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

XynX domains (GH10, CBM9, CBM22) mapped to hydrolase and carbohydrate binding terms

GO_REF:0000003

Gene Ontology annotation based on Enzyme Commission mapping

EC 3.2.1.91 incorrectly assigned to XynX, leading to erroneous cellobiosidase annotation

GO_REF:0000043

Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping

Keywords led to polysaccharide catabolic process and hydrolase activity annotations

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

file:ACET2/P38535/P38535-deep-research-falcon.md

Deep research review of XynX function based on Selvaraj et al. 2010 and other sources

XynX is a GH10 family xylanase, not an exoglucanase

"XynX is a secreted, non-cellulosomal GH10 endo-beta-1,4-xylanase"
The thermostabilizing domain (TSD/CBM22) functions as a xylan-binding domain

"CBM22 primarily mediates xylan binding and contributes to thermostabilization"
XynX binds xylan and lichenan but exhibits negligible cellulase activity

"XynX hardly cleaved cellulosic substrates"
Modular architecture with SLH domains for cell-surface anchoring

"the presence of SLH suggests anchoring to the cell surface S-layer"
Temperature optima range from 50-65 degrees Celsius depending on truncation

"Reported optima for truncations were approximately 60 C (XynX1), 50 C (XynX2), and 65 C (XynX3)"