GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000118

TreeGrafter-generated GO annotations

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

file:PSEPK/gltX/gltX-uniprot.txt

UniProtKB entry for PSEPK gltX

GltX directly aminoacylates tRNA(Glu) with L-glutamate.

"Catalyzes the attachment of glutamate to tRNA(Glu)"

file:PSEPK/gltX/gltX-bioinformatics/RESULTS.md

Sequence assessment of the GltX zinc-binding annotation

The canonical three-cysteine/one-histidine site diverges in KT2440; this does not exclude alternative native zinc binding.

"However, this site-specific divergence is insufficient to exclude all native zinc binding."

PMID:7797500

The zinc-binding site of Escherichia coli glutamyl-tRNA synthetase is located in the acceptor-binding domain. Studies by extended x-ray absorption fine structure, molecular modeling, and site-directed mutagenesis.

The E. coli GluRS canonical site contains three cysteines and one histidine, correcting the legacy four-cysteine interpretation.

"Cys125 and His127"

PMID:25686371

Dispensability of zinc and the putative zinc-binding domain in bacterial glutamyl-tRNA synthetase.

Domain-swapped E. coli constructs separate metal binding from catalytic requirement; native structural absence and a hypothetical target binding capacity are not equivalent.

"whereas the other constructs, all zinc-bound, show impaired function."

PMID:14729703

Direct glutaminyl-tRNA biosynthesis and indirect asparaginyl-tRNA biosynthesis in Pseudomonas aeruginosa PAO1.

Native PAO1 uses direct GlnRS and indirect Asn formation; its GatABC also transamidates supplied heterologous Glu-tRNA(Gln) in vitro. Do not infer native KT2440 Gln-transamidation flux from that capacity.

"The heterotrimeric AdT of P. aeruginosa has no Glu-tRNA Gln substrate in its host, but it can transamidate heterologous Glu-tRNA Gln ."