Gene Ontology annotation reference GO_REF:0000024
Gene Ontology annotation reference GO_REF:0000044
Gene Ontology annotation reference GO_REF:0000117
Getting the mOST from OST: Role of organic solute transporter, OSTalpha-OSTbeta, in bile acid and steroid metabolism.
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Ensembl Compara lists fly FBgn0036834 (CG6836) among putative OST-alpha orthologs. Because invertebrates lack bile acids, the ancestral OST-alpha substrates are proposed to be other molecules, such as steroid hormones or eicosanoids.
"Since bile acids (salts) have not been detected in invertebrate animals [34], these results suggest that the original function of OSTα was to transport non-bile acid (salt) substrates such as steroid hormones or eicosanoids."
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An invertebrate OST-alpha may not need OST-beta, or may use a different partner.
"Alternatively, the invertebrate OSTα orthologue may not require a partner protein or may utilize a different partner protein, and OSTβ evolved more recently as a cofactor for OSTα in vertebrate species."
The heteromeric organic solute transporter, OSTα-OSTβ/SLC51: a transporter for steroid-derived molecules.
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Vertebrate OST-alpha is a seven-TM subunit. It must heterodimerize with OST-beta for stability and for delivery to the plasma membrane. The complex works by facilitated diffusion.
"The transporter functions via a facilitated diffusion mechanism, and can mediate either efflux or uptake depending on the electrochemical gradient of its substrates."
CG6836 family placement analysis (MAFFT alignment and NJ tree of PTHR23423 members)
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CG6836 groups with vertebrate SLC51A, inside an OST-alpha-like clade that also contains the worm osta proteins. It falls outside the TMEM184 and Hfl1-like proteins. Pairwise identity is low (15-21%) and the tree has no bootstrap.
"In the NJ tree CG6836 is sister to the vertebrate SLC51A clade (human, mouse, zebrafish)."
Falcon deep research report for CG6836
NCBP2 modulates neurodevelopmental defects of the 3q29 deletion in Drosophila and Xenopus laevis models.
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RNAi screen of 14 conserved fly homologs of human 3q29-deletion genes for developmental phenotypes and pairwise interactions.
"We systematically characterized developmental, cellular, and nervous system phenotypes for 14 conserved homologs of human 3q29 genes and 314 pairwise interactions using Drosophila"