Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Proteomic study of the Arabidopsis thaliana chloroplastic envelope membrane utilizing alternatives to traditional two-dimensional electrophoresis.
The oligomeric stromal proteome of Arabidopsis thaliana chloroplasts.
The chloroplast lumen and stromal proteomes of Arabidopsis thaliana show differential sensitivity to short- and long-term exposure to low temperature.
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ASP5 abundance responds to cold acclimation.
"identify proteins that
changed in abundance in Arabidopsis chloroplasts"
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Stromule assignment needs target-specific resolution.
Cloning and functional characterization of Arabidopsis thaliana D-amino acid aminotransferase--D-aspartate behavior during germination.
Sorting signals, N-terminal modifications and abundance of the chloroplast proteome.
Hydroponic isotope labelling of entire plants (HILEP) for quantitative plant proteomics; an oxidative stress case study.
Quantitative proteomics of a chloroplast SRP54 sorting mutant and its genetic interactions with CLPC1 in Arabidopsis.
AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins.
Identification of a plant gene encoding glutamate/aspartate-prephenate aminotransferase: the last homeless enzyme of aromatic amino acids biosynthesis.
Analysis of the Arabidopsis cytosolic proteome highlights subcellular partitioning of central plant metabolism.
A proteomic strategy for global analysis of plant protein complexes.
Global Analysis of Membrane-associated Protein Oligomerization Using Protein Correlation Profiling.
Isolation, characterisation and expression of a cDNA clone encoding plastid aspartate aminotransferase from Arabidopsis thaliana.
Recombinant expression, purification, and characterization of three isoenzymes of aspartate aminotransferase from Arabidopsis thaliana.
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Use the directly demonstrated aminotransferase activities.
"Kinetic parameters (Km
and kcat) obtained for all four substrates"
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The enzyme catalyzes aspartate/2-oxoglutarate transamination.
"Kinetic parameters (Km
and kcat) obtained for all four substrates"
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The transaminase directly metabolizes this reaction substrate/product.
"Kinetic parameters (Km
and kcat) obtained for all four substrates"
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Specify the experimentally grounded amino-acid processes.
"Kinetic parameters (Km
and kcat) obtained for all four substrates"
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Retain amyloplast localization as a qualified plastid-subtype assignment.
"asp5 may encode an amyloplastic rather
than the chloroplastic enzyme"
Arabidopsis mutants define an in vivo role for isoenzymes of aspartate aminotransferase in plant nitrogen assimilation.
ASP5 curated UniProt record
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The enzyme catalyzes aspartate/2-oxoglutarate transamination.
"Reaction=L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate;"
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The enzyme acts in plastids, with chloroplast evidence.
"Plastid, chloroplast"
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Pyridoxal phosphate is the integral catalytic cofactor.
"Name=pyridoxal 5'-phosphate;"
Molecular and physiological analysis of Arabidopsis mutants defective in cytosolic or chloroplastic aspartate aminotransferase.