Gene Ontology annotation through association of InterPro records with GO terms
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
3-hydroxypropionyl-coenzyme A dehydratase and acryloyl-coenzyme A reductase, enzymes of the autotrophic 3-hydroxypropionate/4-hydroxybutyrate cycle in the Sulfolobales.
-
The native acryloyl-CoA reductase of M. sedula is encoded by Msed_1426.
"Reinterpretation of the fingerprint mass spectrometry data identified the same gene (Msed_1426) in M. sedula as a best hit (100% identity and 73% coverage)."
-
Recombinant characterization used the 76%-identical S. tokodaii ortholog.
"A gene encoding a protein with 76% amino acid sequence identity to the identified acryloyl-CoA reductase of S. tokodaii is present in the genome of M. sedula (gi 146304192; Msed_1426)."
-
The enzyme uses NADPH, not NADH, and does not reduce crotonyl-CoA.
"The enzyme did not act on either NADH or crotonyl-CoA."
Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate CO(2) fixation cycle in extremely thermoacidophilic archaea.
-
Recombinant M. sedula Msed_1426 was obtained in active form only by co-expression with Msed_1424, and its kinetics were measured.
"ACR could not be expressed in soluble, active form in E. coli, even though several vectors and different expression conditions were tried."
UniProt record A4YGN2 (ACAR_METS5)