Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
The Dictyostelium bZIP transcription factor DimB regulates prestalk-specific gene expression.
-
DimB is a bZIP transcription factor purified by affinity chromatography on ecmA promoter fragments; it spatially restricts ecmA expression rather than being essential for it.
"DimB is not essential for normal expression of the ecmA gene, instead it spatially limits its expression"
-
DimB accumulates rapidly in the nucleus upon DIF-1 exposure and associates with the ecmA promoter in vivo.
"DimB rapidly accumulates in the nucleus when cells are exposed to DIF-1"
bZIP transcription factor interactions regulate DIF responses in Dictyostelium.
-
DimB directly interacts with DimA and both proteins form homo- and heterodimeric complexes in vitro.
"Pull-down assays with purified proteins showed that DimA and DimB can form homodimeric and heterodimeric complexes in vitro"
-
DimB (and DimA) rapidly accumulate in the nucleus after DIF-1 treatment, being uniformly distributed beforehand.
"In the absence of DIF-1, GFP was fairly uniformly distributed throughout the cells. However, after DIF-1 treatment, both DimA:GFP and DimB:GFP exhibited nuclear accumulation"
-
DimB represses non-vacuolar cell death and is required for DimA nuclear accumulation.
"nuclear accumulation of DimA in response to DIF-1 is dependent on DimB"
A new Dictyostelium prestalk cell sub-type.
BTG interacts with retinoblastoma to control cell fate in Dictyostelium.
-
The ALC gene btg is over-induced in dimB-null cells during development, indicating DimB normally represses btg.
"btg is overinduced in the DIF unresponsive mutants"
DIF-1 regulates Dictyostelium basal disc differentiation by inducing the nuclear accumulation of a bZIP transcription factor.
-
DimB is nuclear enriched in pstB cells and directly activates ecmB by binding its promoter, driving basal-disc differentiation.
"ChIP analysis shows that DimB binds to the ecmB promoter in DIF-induced cells"
Transcriptional repression by a bZIP protein regulates Dictyostelium prespore differentiation.
-
DimB binds the pspA promoter and directly represses pspA, while the same pathway activates ecmB.
"Thus DIF induces binding of DimB to the pspA promoter"
The Dictyostelium prestalk inducer differentiation-inducing factor-1 (DIF-1) triggers unexpectedly complex global phosphorylation changes.
Leaps and lulls in the developmental transcriptome of Dictyostelium discoideum.