Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Combined Automated Annotation using Multiple IEA Methods
Bacillus subtilis phage phi29. Characterization of gene products and functions.
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A bulk nonsense-mutant screen of phi29 found that mutants in six cistrons fail to synthesise phage DNA; gene 17 is one of them, as the same laboratory later stated explicitly.
"Mutants in six genes do not synthesize phage DNA."
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The paper also reports a single cistron probably involved in final lysis of the infected bacteria, not two.
"A cistron, probably involved in the final lysis of the infected bacteria, has been found."
Bacteriophage phi29 early protein p17 is conditionally required for the first rounds of viral DNA replication.
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Purified p17 stimulates phi29 DNA amplification in vitro when the reaction starts from a low amount of input DNA.
"A stimulatory effect of protein p17 was demonstrated under in vitro conditions required to amplify phi29 DNA"
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In vivo, p17 is required at low multiplicity of infection, argued to be the natural condition, and dispensable at high multiplicity.
"protein p17 is required when phage infection occurs at a low multiplicity of infection (moi), which is probably the natural condition for infection, but is"
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The authors propose that p17 acts at the very beginning of amplification by recruiting the limiting amount of initiation factors to the replication origins.
"possibly to recruit the limiting amount of initiation factors at the replication origins"
Bacteriophage phi 29 early protein p17. Self-association and hetero-association with the viral histone-like protein p6.
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p17 self-associates into a dimer, with a role proposed for a putative coiled-coil region, and hetero-associates with the viral origin-binding protein p6.
"one hand, on the study of protein p17 dimerization and the role of a putative"
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The authors propose that p17's function is to optimise p6 binding at the viral DNA ends, favouring initiation of replication and negatively modulating p17's own transcription.
"p17 function is to optimize binding of protein p6 at the viral DNA ends, thus"
Factors involved in the initiation of phage phi 29 DNA replication in vitro: requirement of the gene 2 product for the formation of the protein p3-dAMP complex.
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Names the phi29 DNA-synthesis mutants used as extract sources - genes 2, 3, 5, 6 and 17 - which identifies gene 17 as one of the DNA-synthesis cistrons of the 1976 screen.
"extracts from B. subtilis infected with phi29 mutants in genes 2, 3, 5, 6 and 17, involved in DNA synthesis, have been used"
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p17 is not required for formation of the terminal protein p3-dAMP initiation complex, placing its contribution after initiation chemistry.
"The products of genes 5, 6 and 17 do not seem to be needed in the formation of the initiation complex"
Effect of host bacteria genotype on spontaneous reversions of Bacillus subtilis bacteriophage phi29 sus17 nonsense codon.
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Treats gene 17 as an established early gene with a role in phi29 DNA replication, and locates the sus17(112) nonsense codon at the fifth codon of the gene.
"Gene 17 of Bacillus subtilis bacteriophage Phi29 is an early gene playing a role"
The push-pull mechanism of bacteriophage Ø29 DNA injection.
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phi29 DNA enters the host in two steps; in the second, p17 participates in the machinery that pulls the remaining genome into the cell.
"second step at least one of the viral early proteins, p17, participates in the"
Requirements for Bacillus subtilis bacteriophage phi29 DNA ejection.
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The pull step requires an electrochemical proton gradient and negatively supercoiled DNA, and p17 is the component where the energy requirement sits, since azide has no effect in its absence.
"p17 suggests that this protein is essential for energy transduction"
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The second step of ejection is transcription-independent and abolished by novobiocin.
"The second step is transcription-independent and is completely abolished by novobiocin, suggesting a requirement for negatively supercoiled DNA."
Phage phi29 proteins p1 and p17 are required for efficient binding of architectural protein p6 to viral DNA in vivo.
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In cells infected with sus1 or sus17 phage, p6 binding is reduced along the whole phi29 genome, and the authors read the effect as topological, hence indirect.
"proteins p1 and p17 could be required to restrain the proper topology of phi29 DNA"
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It is p1, not p17, that is the membrane-associated phi29 early replication protein proposed to attach the genome to the cell membrane.
"Protein p1 is a membrane-associated viral protein, proposed to attach the viral genome to the cell membrane"
The phage phi29 membrane protein p16.7, involved in DNA replication, is required for efficient ejection of the viral genome.
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p17 is required for efficient internalisation of the left half of the phi29 genome, and its absence affects the pull step more drastically than the absence of p16.7.
"These results corroborate earlier findings that protein p17 is required for efficient ejection of the left half of the φ29 genome"
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p17's replication role is separable from its ejection role: replication is drastically reduced even once the whole sus17 genome has been internalised.
"the absence of p17 was also shown to drastically affect the level of φ29 DNA replication once the entire sus17 genome was internalized"
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Within the phi29 right-side early operon only gene 16.7 encodes a membrane protein, so p17 is not itself membrane-integral.
"Interestingly, 16.7 appears to be the only gene present in the right-side operon to encode a membrane protein"
Molecular phylogeny of phi29-like phages and their evolutionary relatedness to other protein-primed replicating phages and other phages hosted by gram-positive bacteria.
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The gene 17 product is conserved across the phi29-like phages and was used as a phylogenetic marker, described as an accessory component of the phage DNA-replicating machinery.
"terminal proteins and of the gene 17 protein product, an accessory component of"
UniProtKB entry P03686 (GP17_BPPH2), DNA replication protein 17