Gene Ontology annotation through association of InterPro records with GO terms

InterPro domain signatures (IPR000720, IPR008977, IPR000323, IPR036939, IPR014783, IPR014784, IPR020611) identify PAM as a copper type II ascorbate-dependent monooxygenase with catalytic, membrane-associated, and copper-binding properties.

Gene Ontology annotation based on Enzyme Commission mapping

EC 4.3.2.5 maps to GO:0004598 (peptidylamidoglycolate lyase activity), correctly identifying the PAL domain enzymatic activity.

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

UniProt subcellular location "Cytoplasmic vesicle, secretory vesicle membrane" maps to GO:0030658 (transport vesicle membrane).

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

ARBA rule ARBA00093736 identifies PAM as involved in peptide amidation (GO:0001519).

GO_REF:0000118

TreeGrafter-generated GO annotations

PANTHER family assignment (PTN000840922) supports extracellular region localization for PAM based on phylogenetic inference.

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

Combined evidence from EC:1.14.17.3 and PANTHER:PTN000840922 supports peptidylglycine monooxygenase activity (GO:0004504).