Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
The enzymatic component of Drosophila melanogaster chorion is the Pxd peroxidase.
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Recombinant Pxd forms di-tyrosine from tyrosine with H2O2.
"in the presence of H2O2 was able to convert tyrosine residues to di-tyrosine moieties"
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Pxd mRNA is expressed in follicle cells during oogenesis stages 11-14.
"the Pxd mRNA is specifically expressed in follicle cells during the late stages of oogenesis 11-14"
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Pxd protein is in the endochorion and innermost chorionic layer.
"the Pxd peroxidase is selectively localized in the chorion structures and particularly in the endochorion and innermost chorionic layer (ICL)"
Molecular characterization of a putative peroxidase gene of Drosophila melanogaster.
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First cloning of DmPO (Pxd), a single-copy gene at 89D/E encoding a 690-residue protein related to myeloperoxidase and thyroid peroxidase.
"The deduced amino acid sequence reveals an overall homology to myeloeosinophil and thyroid peroxidase"
Crosslinking of theDrosophila chorion involves a peroxidase.
The egg-shell of Drosophila melanogaster III. Covalent crosslinking of the chorion proteins involves endogenous hydrogen peroxide.
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Peroxidase activity and follicle-cell H2O2 are located in the innermost chorionic layer and endochorion, where di- and tri-tyrosine bonds form.
"hydrogen peroxide is an endogenous, programmed product of the follicle cells"
The dual role of chorion peroxidase in Bactrocera oleae chorion assembly.
A structural protein that plays an enzymatic role in the eggshell of Drosophila melanogaster.
The Drosophila drop-dead gene is required for eggshell integrity.
UniProtKB/Swiss-Prot entry Q01603 (PERO_DROME)
Pxd active-site and PANTHER tree / PAINT placement check
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Pxd keeps the catalytic and heme-ligand residues of animal haem peroxidases.
"Q01603 retains His185 (distal His, proton acceptor), Arg331 (transition-state stabiliser) and His437 (proximal heme-iron ligand) at the PROSITE-ProRule PRU00298 positions."
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The only PAINT IBD node Pxd inherits is the root peroxidase-activity node.
"Of the 15 IBD assertions in PTHR11475, Pxd descends from only one: PTN000158755 GO:0004601 peroxidase activity."
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The duplication that separates Pxd's lineage from cardinal and CG42331 is pancrustacean, not fly-specific.
"has taxonomic range Hexapoda-Crustacea in the unfiltered tree"
FlyBase gene description for Pxd (DROME)