Gene Ontology annotation through association of InterPro records with GO terms
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
d-Sedoheptulose-7-phosphate is a common precursor for the heptoses of septacidin and hygromycin B.
-
Recombinant SepB, SepL and SepC reconstruct the ADP-L-glycero-beta-D-manno-heptose pathway of the experimentally identified septacidin cluster.
"SepB, SepL, and SepC can convert
d-sedoheptulose-7-phosphate (S-7-P) to ADP-l-glycero-β-d-manno-heptose"
Original septacidin cluster protein identity analysis
-
Complete sequence comparisons connect current UniProt accessions to the deposited proteins used in the original study.
"SepB A0A2P1G6C0 is identical over all 707 residues to AVM80385.1, the sepB translation in the original MF372757.1 septacidin cluster."
UniProt record for A0A2P1G6C0
Characterization of SepE and SepF for the N(6)-Glycosylated Adenine Structure Formation in Septacidin Biosynthesis.
-
The downstream septacidin glycosyltransferase SepE uses ADP-L-glycero-beta-D-manno-heptose as the sugar donor.
"SepE is an unprecedented
Fe(II)-dependent glycosyltransferase decorating the N6-position of AMP using
ADP-l-glycero-β-d-manno-heptose (6) as a sugar donor."
A conservative distribution of tridomain NDP-heptose synthetases in actinobacteria.
-
Related actinobacterial tridomain proteins can form ADP-D-glycero-beta-D-manno-heptose, but some homologs lack kinase activity.
"ADP-D-glycero-β-D-manno-heptose biosynthesis despite the unexpected discovery of
kinase activities deficient in some proteins."