Annotation inferences using phylogenetic trees
Gene Ontology annotation based on curation of immunofluorescence data
Combined Automated Annotation using Multiple IEA Methods
Identification and characterization of the human peroxin PEX3.
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PEX3 is an integral peroxisomal membrane protein with N-terminus inside peroxisome and C-terminus facing cytoplasm
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PEX19 interacts with PEX3 in mammalian two-hybrid assay
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N-terminal 33 amino acids are necessary and sufficient for peroxisomal targeting
PEX19 binds multiple peroxisomal membrane proteins, is predominantly cytoplasmic, and is required for peroxisome membrane synthesis.
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PEX19 binds a broad spectrum of PMPs including PEX3
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PEX19 is predominantly cytoplasmic with a minor peroxisomal pool
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Loss of PEX19 results in PMP degradation or mislocalization
Defective peroxisome membrane synthesis due to mutations in human PEX3 causes Zellweger syndrome, complementation group G.
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Homozygous PEX3 mutations cause Zellweger syndrome with complete absence of peroxisomal membrane structures
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Wild-type PEX3 expression restores peroxisomal biogenesis in mutant cells
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PEX3 is established as a key factor in early human peroxisome synthesis
Two splice variants of human PEX19 exhibit distinct functions in peroxisomal assembly.
Analysis of mammalian peroxin interactions using a non-transcription-based bacterial two-hybrid assay.
The interaction between human PEX3 and PEX19 characterized by fluorescence resonance energy transfer (FRET) analysis.
PEX3 functions as a PEX19 docking factor in the import of class I peroxisomal membrane proteins.
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PEX3 is the membrane docking factor for PEX19
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PEX3 depletion specifically blocks class I PMP import
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PEX3 is sufficient to dock PEX19 at heterologous organelles
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Conserved motif aa120-136 of PEX3 is essential for PEX19 binding
Towards a proteome-scale map of the human protein-protein interaction network.
In vitro transport of membrane proteins to peroxisomes by shuttling receptor Pex19p.
Characterization of the interaction between recombinant human peroxin Pex3p and Pex19p: identification of TRP-104 IN Pex3p as a critical residue for the interaction.
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PEX3-PEX19 form a 1:1 complex with KD of 3.4 nM
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Trp-104 of PEX3 is critical for PEX19 binding
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PEX19 binding mutations impair peroxisome restoring activity
Pex3p-dependent peroxisomal biogenesis initiates in the endoplasmic reticulum of human fibroblasts.
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PEX3 transits through the ER before reaching peroxisomes during de novo biogenesis
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PEX3 requires PEX16 for ER localization
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PEX3 can drive peroxisome biogenesis from the ER
The cytosolic domain of PEX3, a protein involved in the biogenesis of peroxisomes, binds membrane lipids.
Defining the membrane proteome of NK cells.
Structural basis for docking of peroxisomal membrane protein carrier Pex19p onto its receptor Pex3p.
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Crystal structure of PEX3-PEX19 complex at 2.50 A resolution
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PEX3 has a novel twisted six-helix bundle fold
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PEX19 binds at apex of PEX3 via an alpha-helix with leucine triad motif
PEX14 is required for microtubule-based peroxisome motility in human cells.
Sec16B is involved in the endoplasmic reticulum export of the peroxisomal membrane biogenesis factor peroxin 16 (Pex16) in mammalian cells.
A proteome-scale map of the human interactome network.
A massively parallel pipeline to clone DNA variants and examine molecular phenotypes of human disease mutations.
Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Architecture of the human interactome defines protein communities and disease networks.
LuTHy: a double-readout bioluminescence-based two-hybrid technology for quantitative mapping of protein-protein interactions in mammalian cells.
Maximizing binary interactome mapping with a minimal number of assays.
Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.
A reference map of the human binary protein interactome.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
OpenCell: Endogenous tagging for the cartography of human cellular organization.
AI-guided pipeline for protein-protein interaction drug discovery identifies a SARS-CoV-2 inhibitor.
Systematic discovery of protein interaction interfaces using AlphaFold and experimental validation.
Multimodal cell maps as a foundation for structural and functional genomics.
Cloning and characterization of the gene encoding the human peroxisomal assembly protein Pex3p.
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First cloning of human PEX3
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PEX3 localized to peroxisomes by immunofluorescence
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N-terminal 40 amino acids sufficient for targeting
Peroxisome synthesis in the absence of preexisting peroxisomes.
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PEX3, PEX16, PEX19 are involved in peroxisome membrane synthesis
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Peroxisomes can form de novo
PEX-19 docks ABCD1/D2/D3 to peroximal membrane
PEX3:PEX19:class I PMP dissociates
PEX19:class I PMP binds PEX3