Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
UniProtKB entry Q02318 (CP27A_HUMAN), Sterol 26-hydroxylase, mitochondrial
Putative helix F contributes to regioselectivity of hydroxylation in mitochondrial cytochrome P450 27A1.
Metabolism of 4 beta -hydroxycholesterol in humans.
Metabolism of vitamin D by human microsomal CYP2R1.
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The vitamin D3 comparison finds greater CYP2R1 catalytic efficiency; it does not negate the measured CYP27A1 D3 hydroxylase activity or show that activity is nonphysiological.
"The k(cat)/K(m) value of CYP2R1 was 26-fold higher than that of CYP27A1."
Characterization of human sterol 27-hydroxylase. A mitochondrial cytochrome P-450 that catalyzes multiple oxidation reaction in bile acid biosynthesis.
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Human CYP27A1 expressed with adrenodoxin catalyzes successive terminal sterol-side-chain oxidations, supporting its contribution to bile-acid-intermediate formation.
"multiple oxidation reactions (R-CH3----R-CH2OH----R-COOH) at carbon 27 of sterol intermediates"
Mutations in the bile acid biosynthetic enzyme sterol 27-hydroxylase underlie cerebrotendinous xanthomatosis.
Conversion of 7-ketocholesterol to oxysterol metabolites by recombinant CYP27A1 and retinal pigment epithelial cells.
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Reconstituted human CYP27A1 converts 7-ketocholesterol to side-chain oxygenated products, with higher in-vitro turnover than cholesterol under the tested conditions.
"7KCh is metabolized at a 4-fold higher rate"
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Novel homozygous and compound heterozygous mutations of sterol 27-hydroxylase gene (CYP27) cause cerebrotendinous xanthomatosis in three Japanese patients from two unrelated families.
Activities of recombinant human cytochrome P450c27 (CYP27) which produce intermediates of alternative bile acid biosynthetic pathways.
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Recombinant human CYP27A1 hydroxylates cholesterol and further oxidizes the alcohol to aldehyde and acid products.
"further oxidize 27-hydroxycholesterol giving first an aldehyde and then 3beta-hydroxy-5-cholestenoic acid"
A novel Arg362Ser mutation in the sterol 27-hydroxylase gene (CYP27): its effects on pre-mRNA splicing and enzyme activity.
CYP27A1 27-hydroxylates 5bCHOL3a,7a,12a-triol
5beta-cholestan-3alpha,7alpha,12alpha,27-tetrol is oxidized to 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-27-al
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-27-al is oxidized to 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate (THCA)
CYP27A1 27-hydroxylates CHOL
Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
5beta-cholestan-3alpha, 7alpha-diol is hydroxylated to 5beta-cholestan-3alpha, 7alpha, 26-triol
3alpha, 7alpha-dihydroxy-5beta-cholestan-26-al is oxidized to 3alpha, 7alpha-dihydroxy-5beta-cholestanoate (DHCA)
5beta-cholestan-3alpha, 7alpha, 26-triol is oxidized to 3alpha, 7alpha-dihydroxy-5beta-cholestan-26-al
5beta-cholestan-3alpha,7alpha,24(S),27-tetrol is oxidized to 3alpha,7alpha,24(S)-trihydroxy-5beta-cholestan-27-al
3alpha,7alpha,24(S)-trihydroxy-5beta-cholestan-27-al is oxidized to 3alpha,7alpha,24(S)-trihydroxy-5beta-cholestanoate (3,7,24THCA)
Synthesis of bile acids and bile salts via 24-hydroxycholesterol
CYP27A1 27-hydroxylates 5β-CHOL3α,7α,24(s)-triol
Synthesis of bile acids and bile salts via 27-hydroxycholesterol
CYP27A1 does not 27-hydroxylate 5bCHOL3a,7a,12a-triol
Defective CYP27A1 does not 27-hydroxylate 5β-CHOL3α,7α,24(s)-triol
Mitochondrial targeting of cytochrome P450 proteins containing NH2-terminal chimeric signals involves an unusual TOM20/TOM22 bypass mechanism.
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Rat CYP27A1 expressed in yeast mitochondria is protected inside the inner-membrane matrix compartment and is mostly membrane-extrinsic by carbonate extraction.
"they are mostly membrane extrinsic proteins"
Cilia-Associated Oxysterols Activate Smoothened.
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Mouse Ptch1-null MEF depletion and downstream-oxysterol rescue support CYP27A1 participation in producing Smoothened-activating oxysterols.
"shRNA-mediated depletion of CYP27A1 inhibited HH pathway activity"
A cDNA encoding a rat mitochondrial cytochrome P450 catalyzing both the 26-hydroxylation of cholesterol and 25-hydroxylation of vitamin D3: gonadotropic regulation of the cognate mRNA in ovaries.
Transfected human liver cytochrome P-450 hydroxylates vitamin D analogs at different side-chain positions.
Cytochrome P450 27A1 Deficiency and Regional Differences in Brain Sterol Metabolism Cause Preferential Cholestanol Accumulation in the Cerebellum.
CYP27A1 source and assay guide