Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Cloning, expression, and characterization of two manganese superoxide dismutases from Caenorhabditis elegans.
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Cloned sod-2 and sod-3, showed both encode mitochondrial (transit-peptide bearing) manganese-type superoxide dismutases (~86% identical proteins), and directly measured their enzymatic activity after heterologous expression of the mature chains in SOD-deficient E. coli (Mn-type: insensitive to hydrogen peroxide and cyanide; dimeric; protective against methyl-viologen/paraquat oxidative stress). Both proteins were assayed, so the biochemical evidence is sod-3-specific as well as sod-2-specific.
Novel interactions between mitochondrial superoxide dismutases and the electron transport chain.
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SOD-3 (like SOD-2) localizes to the mitochondrial respiratory supercomplex I:III:IV by blue-native gel Western. In contrast to sod-2, loss of sod-3 does not decrease complex I-dependent respiration and sod-3 BNGs are normal; sod-3 single-mutant lifespan equals wild type, but sod-3 has distinct, non-redundant genetic interactions with the ETC mutants gas-1, mev-1 and isp-1. sod-3 is an mtSOD normally expressed at very low levels and induced by DAF-16.
The MAP kinase JNK-1 of Caenorhabditis elegans: location, activation, and influences over temperature-dependent insulin-like signaling, stress responses, and fitness.
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Establishes sod-3 as a DAF-16 target gene whose peripheral, non-neuronal expression is promoted by JNK-1-driven nuclear translocation of DAF-16. UniProt cites this paper for the SUBCELLULAR LOCATION (mitochondrion), TISSUE SPECIFICITY (pharynx/rectum basally; vulva, body-wall muscle, hypodermis on thermal stress), and heat INDUCTION of SOD-3.