Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
Pseudomonas aeruginosa mutants affected in anaerobic growth on arginine: evidence for a four-gene cluster encoding the arginine deiminase pathway.
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Mutants unable to grow anaerobically on arginine include arcA mutants lacking arginine deiminase, distinct from regulatory arc mutants with low levels of all three enzymes.
"The mutations arcA (arginine deiminase), arcB (catabolic ornithine carbamoyltransferase), arcC (carbamate kinase), and arcD were highly cotransducible and mapped in the 17-min chromosome region."
Sequence analysis and expression of the arginine-deiminase and carbamate-kinase genes of Pseudomonas aeruginosa.
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The arcA ORF encodes the 46 kDa ADI subunit (matching purified enzyme size and N-terminal sequence); native ADI is a tetramer with Km 0.04 mM for arginine, activated by Mg2+/Mn2+ and inhibited by Zn2+.
"The enzyme was activated by Mg2+ or Mn2+ and strongly inhibited by Zn2+."
The arc operon for anaerobic arginine catabolism in Pseudomonas aeruginosa contains an additional gene, arcD, encoding a membrane protein.
Structural insight into arginine degradation by arginine deiminase, an antibacterial and parasite drug target.
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Crystal structure of P. aeruginosa ADI; pentein fold shared with DDAH and amidinotransferases; conserved Cys-His-Asp catalytic motif.
"We have determined the crystal structure of ADI from Pseudomonas aeruginosa by the multi-wavelength anomalous diffraction method at 2.45 A resolution."
Crystal structures representing the Michaelis complex and the thiouronium reaction intermediate of Pseudomonas aeruginosa arginine deiminase.
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Cys406 is the catalytic nucleophile forming an S-alkylthiouronium intermediate; His278, Asp280 and Asp166 mediate proton transfers.
"The function of Cys406 in nucleophilic catalysis has been demonstrated by transient kinetic studies."