Human GC1 catalytic activity was measured directly despite the GCAP3-focused study title PMID:35328663.
All 49 source contexts have individual manual decisions. The genuine private seeder produced 48 annotations; the distinct cone-cell extension was restored as an original assertion. The raw archive preserves both original records and dates. Both extension predicates use the schema CURIE BFO:0000050.
The whole review retains the three peered manual slices, with one approved specificity refinement: raw43 uses the same unextended located_in comparator as raw44. The original positive localization evidence remains in the source guide. File citations point to independent phylogeny and source proofs rather than the incoming annotation archive.
The current consistency table does not compare UNDECIDED with ACCEPT or REMOVE. Source-specific and extension-specific uncertainty stays explicit in the reasons; inactive consistency_exception objects are omitted.
No provider-generated research file is claimed. The reviewed private primary readings, official data and independent peers support this proposal. Existing normal sources remain unchanged; genuine native acquisition, product reconciliation, history, validation and render results will be appended after execution.
Genuine native helpers completed the 48 missing source records and preserved all five inherited source records byte-for-byte. The genuine 48-row seed and original machine records matched the frozen inputs; the separately preserved cone-cell context was restored, retaining all 49 incoming contexts and both extension atoms. Genuine product declarations were retained. Titles and cache availability flags now match the actual normal records; independent original-reading limits remain separately documented. History, validation and rendering results follow after execution.
Gene, reference, genuine CREATE-history and family checks passed. The reference validator checked one excerpt and 48 titles with no issues. The actual materialized family corpus at the native base had zero conflicts; unrelated unresolved entries remain reported. Computed review status remains DRAFT with two legacy same-term advisories for the deliberately distinct source and extension contexts. No action was changed to obtain a status. HTML was regenerated; original contexts and source bytes were preserved. Publication and later exact-head review and CI remain separate.
RetGC1 supplies photoreceptor cGMP; GCAP-dependent calcium feedback supports response recovery [PMID:35328663; UniProtKB:Q02846].
The original rat immunogold caption specifies “disk and plasma membranes within the same rod outer segment” [PMID:9153227, Figure 5; original indexed text].
The bovine study reports positive phosphotransferase activity but states “mutagenesis of the kinase domain is needed” for definitive intrinsic attribution [PMID:8900199, Discussion].
Tested natriuretic nonresponse does not establish absence of every peptide response; the broad receptor and extracellular-initiation claims remain unresolved PMID:1356371.
Transfected-cell ER staining does not independently demonstrate endogenous ER-membrane residence PMID:30319355.
Original-access URLs and precise sections are recorded in the source evidence. Cache availability flags describe the unchanged normal records. Historical preparation entries above remain provenance; this follow-up adds biological interpretation without inventing a provider-generated research file.
The genuine record-section helper added three unambiguous sections. Gene, reference, strict GOA and genuine EDIT-history checks passed. The reference validator checked seven excerpts and 48 titles with no issues; three local-record excerpts were skipped by that validator and were independently checked in the frozen peer. Existing status was reconciled to the repository-computed COMPLETE with no validation warnings; source-specific uncertainties remain in their reviewed decisions. All 49 incoming contexts, both extension atoms, product declarations and 53 normal sources remain preserved. HTML was regenerated. Exact-head review and CI remain separate publication gates.
Validation added the previously absent GO:0042622 term-cache row. Its exact genuine content was independently checked and retained; no normal publication, pathway or family source was changed.
The electronic nuclear-envelope assignment is removed as an unsupported compartment prediction; the rule’s training provenance remains unknown [ARBA:ARBA00086599].
The four reviewed files were applied after a separately completed existing-branch transition. Gene, strict GOA, reference and genuine EDIT-history checks passed. The reference check verified seven excerpts and 48 titles with no issues; three local-record excerpts retain their independent verification. Computed status remains COMPLETE with no validation warnings. HTML was regenerated and the final sorted-cache check passed. All 53 normal sources and the existing ontology cache remain byte-exact. Publication, current-head review and CI remain separate gates.
Human GC1 and human RD3 specifically coprecipitated in transfected cells [PMID:21078983, Methods and Figure 2C].
Human GC1 coprecipitated with mouse Ankmy2, with ABCA4 as a negative control [PMID:21124868, Methods and Figure 6].
The original full-text Methods and Results were rechecked in the unchanged publication caches and their PMC3000275 and PMC2991253 originals. Stored rows 14 and 15 now retain these interactions as KEEP_AS_NON_CORE, following the standing ClinGen project instruction. Their previous removal was solely informational. No evidence-backed specific replacement has been established. The prepared compartment correction and the five unresolved decisions remain unchanged.
Direct validation with the retained runtime passed schema, ontology terms, reference excerpts and titles, strict GOA matching, and history checks. The reference validator checked nine excerpts and 48 titles with no issues; three local-record excerpts remain independently verified. The only two best-practice warnings are the generic-binding advisories superseded by the project instruction. The computed status is therefore recorded as DRAFT; all five UNDECIDED reviews remain explicit. HTML was regenerated.