Gene Ontology annotation through association of InterPro records with GO terms
-
CelA carries InterPro records IPR002037 (Glyco_hydro_8), IPR008928 (6-hairpin_glycosidase_sf), IPR012341 (6hp_glycosidase-like_sf), and IPR002105/IPR016134/IPR036439 (Dockerin), which drive the InterPro2GO inferences of carbohydrate metabolism and polysaccharide catabolism.
Gene Ontology annotation based on Enzyme Commission mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
-
UniProtKB keyword KW-0136 (Cellulose degradation) maps to GO:0030245 (cellulose catabolic process).
-
UniProtKB keyword KW-0326 (Glycosidase) maps to GO:0016798 (hydrolase activity, acting on glycosyl bonds).
-
UniProtKB keyword KW-0378 (Hydrolase) maps to GO:0016787 (hydrolase activity).
Combined Automated Annotation using Multiple IEA Methods
Sequence of a cellulase gene of the thermophilic bacterium Clostridium thermocellum
-
Original cloning and sequencing of the celA gene encoding Endoglucanase A
"The nucleotide sequence of the celA gene, encoding the extracellular endoglucanase A of Clostridium thermocellum, was determined and compared with the NH2-terminal amino acid sequence of the purified enzyme"
The crystal structure of endoglucanase CelA, a family 8 glycosyl hydrolase from Clostridium thermocellum
-
Crystal structure at 1.65 A resolution confirms GH8 family fold with active site cleft
"The protein folds into a regular (alpha/alpha)6 barrel formed by six inner and six outer alpha helices. Cello-oligosaccharides bind to an acidic cleft containing at least five D-glucosyl-binding subsites"
Atomic (0.94 A) resolution structure of an inverting glycosidase in complex with substrate
-
High-resolution structure reveals active site and catalytic mechanism
"The crystal structure of Clostridium thermocellum endoglucanase CelA in complex with cellopentaose has been determined at 0.94 A resolution. The oligosaccharide occupies six D-glucosyl-binding subsites"
Functional subgenomics of Clostridium thermocellum cellulosomal genes: identification of the major catalytic components in the extracellular complex and detection of three new enzymes.
-
CelA/Cel8A identified as one of the major catalytic components of the cellulosome
"Ten of the components were previously known: the structural protein CipA, the endo-glucanases Cel8A, Cel5G, Cel9N, the cellobiohydrolases Cbh9A, Cel9K, Cel48S, the xylanases Xyn10C, Xyn10Z, and the chitinase Chi18A"
-
Seventy-one putative cellulosomal genes detected in C. thermocellum genome
"Seventy-one putative cellulosomal genes were detected. One third of these ORFs may be involved in cellulose hydrolysis"
Global view of the Clostridium thermocellum cellulosome revealed by quantitative proteomic analysis
-
Quantitative proteomic analysis of cellulosome composition
"In total, 41 cellulosomal proteins were detected, including 36 type I dockerin-containing proteins, which count among them all but three of the known docking components and 16 new subunits"
Enzymatic degradation of cellulose in soil - A review
-
2024 review describing cellulosome architecture including CelA as endoglucanase component
"the anchoring of various cellulolytic enzymes, i.e., endoglucanases, cellobiohydrolases, xylanases, to a scaffoldin makes it a complex structure in which different enzymes work synergistically to attack heterogeneous, insoluble cellulose substrates"
Deep research review of celA gene function
-
Comprehensive literature synthesis on CelA function and cellulosome localization
"CelA/Cel8A is an endo-beta-1,4-glucanase (EC 3.2.1.4) that cleaves internal bonds in cellulose chains, producing new chain ends for exoglucanases. Structural evidence shows a groove/open cleft typical of endo-acting enzymes"