Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Combined Automated Annotation using Multiple IEA Methods
The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification.
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Immunopurification and mass spectrometry of human NADH dehydrogenase resolved and identified the human homologues of 42 Complex I polypeptides, including NDUFB9/B22, establishing NDUFB9 as a component of the complex.
"we can resolve and identify the human"
Towards a proteome-scale map of the human protein-protein interaction network.
Huntingtin interacting proteins are genetic modifiers of neurodegeneration.
A proteome-scale map of the human interactome network.
Accessory subunits are integral for assembly and function of human mitochondrial complex I.
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Systematic gene-editing knockouts of each human Complex I accessory subunit showed that most accessory subunits are strictly required for assembly of a functional complex, and that loss of a subunit destabilizes other subunits of the same structural module.
"required for assembly of a functional complex and 1 subunit is essential for"
Architecture of the human interactome defines protein communities and disease networks.
Architecture of Human Mitochondrial Respiratory Megacomplex I(2)III(2)IV(2).
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Cryo-EM structure of the human respiratory megacomplex I2III2IV2 enabled precise assignment of individual subunits of human Complex I (including NDUFB9) and confirms its position within the inner-membrane complex and supercomplex.
"precise assignment of individual subunits of"
Assembly of mammalian oxidative phosphorylation complexes I-V and supercomplexes.
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Review of OXPHOS assembly noting that the human complexes comprise catalytic core proteins plus a large number of supernumerary subunits that play essential roles in assembly, regulation and stability.
"supernumerary' subunits that play essential roles in assembly, regulation"
A reference map of the human binary protein interactome.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
The human B22 subunit of the NADH-ubiquinone oxidoreductase maps to the region of chromosome 8 involved in branchio-oto-renal syndrome.
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Identified the human B22 (NDUFB9) subunit as a mitochondrial protein of the respiratory electron transport chain and, because it maps to the 8q13 BOR region, proposed it as a candidate for hearing loss — a positional candidacy, not a demonstrated auditory function.
"a mitochondrial protein in the respiratory electron transport chain"
cDNA of eight nuclear encoded subunits of NADH:ubiquinone oxidoreductase: human complex I cDNA characterization completed.
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Characterized cDNAs completing the set of nuclear-encoded human Complex I subunits; describes the complex's function as transport of electrons from NADH to ubiquinone accompanied by proton translocation.
"the transport of electrons from NADH to ubiquinone, which is"
Complex I oxidises NADH to NAD+, reduces CoQ to CoQH2
Intermediate 1 binds HP subcomplex to form Intermediate 2
Peripheral arm subunits bind the 815kDa complex to form a 980kDa complex
Intermediate 2 binds MT-ND1:NDUFAF5:NDUFAF6 to form a 315kDa subcomplex
The MCIA complex, NDUFAF2-7 all dissociate from the 980kDa complex, resulting in Complex I
ND4, ND5 bind the 550kDa complex to form the 815kDa complex
The 315kDa subcomplex binds the 370kDa subcomplex to form the 550kDa complex