Human GANAB is the catalytic alpha subunit of glucosidase II. The selected original human experiments support two long splice forms with PRKCSH and distinguish both inner-glucose-removal steps from MOGS's outermost-glucose reaction (PMID:10929008(https://doi.org/10.1093/glycob/10.8.815)). The current specific GO term names only Glc2-to-Glc1; the second step remains explicit biological prose in the same catalytic core. The polymer-glucan term is corrected on substrate chemistry, not on a purported phylogenetic loss.
Disease/client work supports impaired polycystin maturation and trafficking when GANAB is lost, without demonstrating GANAB as a ciliary resident or ion transporter (PMID:27259053(https://doi.org/10.1016/j.ajhg.2016.05.004), PMID:28375157(https://doi.org/10.1172/JCI90129)). The latter paper's official corrigendum corrects Pkd1 primer labeling; no blanket invalidation of its GANAB claims follows. WT localization and engineered-mutant distribution must remain distinct (PMID:33097077(https://doi.org/10.1186/s13023-020-01585-4)).
The three current protein sequences are 944/966/52 aa. The first two correspond to experimentally supported long alpha forms. The 52-aa product has no demonstrated catalytic function; sequence existence does not settle endogenous protein abundance. Golgi inference traces to the actual pig donor P79403, while target ER staining is supported by HPA and human construct work. Root ER annotations are retained beside lumen specificity.
The original target-table joins provide positive melanosome/vesicle and HeLa RNA-contact evidence, with their actual purification and identification limits. These observations are contextual and do not create a new core or inferred biological process. The precise 19946888 membrane-fraction claim remains unresolved because the target table was not recovered; a failed access route is not a negative biological experiment. See the portable source guide and external evidence for stable locators and exact target rows.
All 39 original contexts are preserved in this private proposal; raw 13/14 retain their distinct source metadata despite a forecast native collision. The future native stage must reconcile a genuine seed and restore the second original, preserving both original extension atoms. No NEW annotation is proposed. No canonical review, source cache, branch or native history has been changed at this stage. A source-specific quote ledger distinguishes already available immutable-cache anchors from two official-abstract anchors awaiting genuine 10929008 cache verification.
Missing-only acquisition reused 25 immutable main sources and acquired 9 absent sources. The actual 39-row, 16-column export matches the preserved official contexts exactly; genuine seeding yields the expected 38 rows. The two distinct 10929008/GO0006491 source records are restored as 39 originals, with no NEW assertion. All three product records and both urine/saliva extension atoms are preserved. All five anchors pass exact native or portable-source checks; two 10929008 snippets require only source-verbatim line breaks, preserving their words and aggregate budget. The 27259053 title is normalized to the native cache and full-text availability is recorded without broadening the selected reading scope. The untouched PENDING seed, raw export and UniProt record remain preserved. A native codex CREATE history records the transfer. Default validation passed with zero curation advisories. Strict GOA matched all current originals, native history passed, and the status audit computes COMPLETE while the source-specific membrane judgment remains UNDECIDED. Private rendering, links and scoped whitespace checks passed.
The initial same-core prose for final-glucose hydrolysis is superseded by an explicit ontology proposal and a second substrate-specific core. GO:0106407 describes Glc2-to-Glc1 hydrolysis; the proposed Glc1Man9GlcNAc2 oligosaccharide glucosidase activity describes the next reaction, supported by the selected original human assays, UniProt RHEA:56000 and the normal Reactome final-glucose event (PMID:10929008(https://doi.org/10.1093/glycob/10.8.815); R-HSA-548890). Five existing MODIFY recommendations now include both specific reaction terms. Original evidence, qualifiers, actions and source records are preserved; no NEW row is added.
The already verified pig P79403 Golgi chain now has a normal PMID:2404989 cache, manual reference assessment and explicit support links in both human Golgi rows (PMID:2404989(https://doi.org/10.1083/jcb.110.2.309)). The abstract reports kidney tubular post-Golgi localization; species is identified by the official donor record. This remains contextual ortholog evidence, not direct human imaging. The donor reverse-ISS melanosome chain is not substituted for this evidence.
The broad activity row keeps the 28375157 citation and client-biogenesis context but drops its client-phenotype quote, relying on 10929008 for specific chemistry. The two process reasons now name ComplexPortal and UniProt rather than internal raw indices, which remain in the original-context archive. RNA contact retains the method-specific ion-count versus DESeq distinction (adjusted P=0.930) and ER-lumen topology limit; neither fact proves an artifact. Ancillary carbohydrate binding remains non-core, with no separate lectin function asserted.
One missing source was acquired without force after bounded owner checks. All prior 34 source bytes and 39 original machine records remain exact. A native codex EDIT records the amendment. Default validation passed with zero curation advisories. Strict GOA matches all 39 original contexts; native codex EDIT history, computed COMPLETE status, private rendering, links and scoped whitespace checks passed. The one source-specific UNDECIDED judgment is retained.
The Golgi ISS reason and donor comment now attribute only the post-Golgi/brush-border distribution to the PMID:2404989 abstract. The official pig donor record remains the basis for the Golgi inference, and the non-core judgment is unchanged. This separates the abstract wording from the curated donor location without adding a direct human assay.
The evidence archive stage now reports the native checks already recorded as completed in its detailed results; two spacing errors are corrected. All 39 original annotations, actions, three products, two cores, five quotations and 35 source files are unchanged. This follow-up adds no evidence quotation. A native codex EDIT records the follow-up. Default validation passed with zero curation advisories. Strict GOA matches all 39 original contexts; native codex EDIT history, computed COMPLETE status, private rendering, links and scoped whitespace checks passed. The one source-specific UNDECIDED judgment is retained.
The renewed review identified production usage missed by a human enabled-by index search. The actual FlyBase model contains two causally linked Gcs2alpha activities typed GO:0106407, each with Q14697 in its enabled_by ISS evidence. Their sequence between glucosidase I and mannosidase, read alongside the human Glc1/Glc2 experiments (PMID:10929008(https://doi.org/10.1093/glycob/10.8.815)), supports the two-step interpretation. The model does not explicitly label the intermediate glycans, and its ER-lumen donor is separately P38138. The combined fly module and retained ec2go-derived EC mapping document similar use of the existing term; neither is an additional primary enzyme experiment.
This positive usage replaces the earlier null-search rationale. Reconciling the existing GO:0106407 definition is preferred because it preserves current model/module typing. The final-glucose NTR remains conditional: a separate term would require retyping the appropriate downstream activity and separating the final step within the combined module, not replacing both activities indiscriminately. The two cores and five replacement lists remain intact.
The actual GO:0106407 is_a GO:0090599 edge supports the stated refinement. Product-anomer wording is a separate unresolved issue: the official GH31 family description describes a retaining mechanism, whereas the curated RHEA products are beta-D-glucose. The conditional definition now uses D-glucose and a question requests reconciliation; no direct product-anomer measurement or source defect is claimed. The existing first-core excerpt supports the two-step chemistry and is reused by reference rather than duplicated. All source bytes, original contexts, actions, products and quotations remain unchanged. A native codex EDIT records the follow-up. Default validation passed with zero curation advisories. Strict GOA matches all 39 original contexts; native codex EDIT history, computed COMPLETE status, private rendering, links and scoped whitespace checks passed. The one source-specific UNDECIDED judgment is retained.