Gene Ontology annotation through association of InterPro records with GO terms

InterPro signatures support family-based chemistry and autophagy-regulation assertions.

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

Curated sequence-similarity transfer supplies the mouse-derived outer-mitochondrial-membrane assertion.

GO_REF:0000033

Annotation inferences using phylogenetic trees

PAINT supplies inherited mitochondrial-autophagy, outer-membrane and cluster-binding assertions.

GO_REF:0000052

Gene Ontology annotation based on curation of immunofluorescence data

HPA immunofluorescence supplies direct ER localization.

GO_REF:0000054

Gene Ontology annotation based on curation of intracellular localizations of expressed fusion proteins in living cells

LIFEdb supplies a historical fusion-protein complex-location assertion.

GO_REF:0000107

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara

Ensembl transfer supplies the mouse-linked mitochondrial-autophagy assertion.

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

ARBA supplies broad cytoplasmic localization.

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

Combined electronic sources supply membrane locations and iron–sulfur binding.

PMID:17376863

MitoNEET is an iron-containing outer mitochondrial membrane protein that regulates oxidative capacity.

Tagged human Miner1 localizes to a perinuclear reticular ER pattern; the paper's detailed mitochondrial import and membrane experiments concern mitoNEET.

PMID:19580816

Crystal structure of Miner1: The redox-active 2Fe-2S protein causative in Wolfram Syndrome 2.

Human soluble Miner1 forms a homodimer with one [2Fe-2S] cluster per protomer and has measurable cluster redox potential and lability.

PMID:19946888

Defining the membrane proteome of NK cells.

The study surveys membrane proteins in a human NK-cell preparation.

PMID:20010695

Antagonism of Beclin 1-dependent autophagy by BCL-2 at the endoplasmic reticulum requires NAF-1.

CISD2 associates with BCL2, BCLXL and IP3R1 and helps BCL2 restrain Beclin 1-dependent starvation autophagy at the ER.

PMID:22658674

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.

CISD2 is a statistically enriched HeLa mRNA-interactome protein in the original supplement.

PMID:32296183

A reference map of the human binary protein interactome.

HuRI reports binary interactions for CISD2 with multiple screened human protein constructs.

PMID:32814053

Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.

The neurodegeneration interactome includes positive CISD2 yeast two-hybrid pairs.

PMID:23717386

Nutrient-deprivation autophagy factor-1 (NAF-1): biochemical properties of a novel cellular target for anti-diabetic drugs.

Purified soluble human NAF-1 delivers its bound cluster to apoferredoxin in a redox- and ligand-sensitive reaction.

PMID:24706857

Integrated strategy reveals the protein interface between cancer targets Bcl-2 and NAF-1.

BCL2 BH4 and BH3 peptides bind soluble NAF-1, and a BH4 peptide destabilizes its cluster and accelerates transfer to apoferredoxin.

PMID:19451219

Cisd2 deficiency drives premature aging and causes mitochondria-mediated defects in mice.

Mouse Cisd2 deficiency causes mitochondrial degeneration, increased autophagy and premature aging; endogenous fractionation supports an outer-mitochondrial-membrane pool.

PMID:17846994

A homozygous mutation in a novel zinc-finger protein, ERIS, is responsible for Wolfram syndrome 2.

Human CISD2 variants disrupt splicing in Wolfram syndrome 2, and tagged human ERIS localizes to the ER.

PMID:39370046

CISD2 counteracts the inhibition of ER-mitochondrial calcium transfer by anti-apoptotic BCL-2.

Cytosolic CISD2 binds purified BCL2 BH4, while its effects on BCL2-associated calcium signaling differ between bulk release and ER–mitochondrial contacts.

file:human/CISD2/CISD2-source-evidence.json

CISD2 source identities, assay scope and access ledger

Original assertions, exact interaction records, donor identities and source-specific access are preserved as audit provenance.