Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in gram-negative bacteria.
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Identified the chromosomal arsRBC operon as arsenite-inducible and required for arsenite and arsenate resistance.
"Mutants in the chromosomal ars operon were found to be approximately 10- to 100-fold more sensitive to sodium arsenate and arsenite exposure than wild-type E. coli"
The chromosomal arsR gene of Escherichia coli encodes a trans-acting metalloregulatory protein.
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Purified ArsR binds a 33-nt operator over the -35 element and represses the chromosomal ars operon in trans; cross-regulates with R773 ArsR.
"the chromosomally-encoded repressor can regulate expression of the ars operon of plasmid R773, and the R773 repressor can cross-regulate expression from the chromosomal operon"
Expression of the Escherichia coli chromosomal ars operon.
Dimerization is essential for DNA binding and repression by the ArsR metalloregulatory protein of Escherichia coli.
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ArsR is a homodimer; dimerization is required for promoter binding and inducer response; an ~80-residue core suffices.
"These results suggest that a core sequence of about 80 residues has all of the information necessary for dimerization, repression, and metal recognition."
The role of arsenic-thiol interactions in metalloregulation of the ars operon.
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In R773 ArsR, As(III) is coordinated by Cys32, Cys34 and Cys37 (AsS3); Cys32 and Cys34 are required for induction. The same cysteine motif is present in K-12 ArsR (ELCVCDLC).
"These results suggest that all three cysteine thiolates are arsenic ligands"
Beyond Repression: ArsR Functions as a Global Activator of Metabolic and Redox Responses in Escherichia coli.
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Proteomics of arsRBC-deleted vs arsR-complemented K-12 suggests broader, possibly indirect, ArsR-dependent effects on metabolic and redox proteins.
"Our study shows that ArsR influences proteomic networks beyond the ars operon"
The Chromobacterium violaceum ArsR Arsenite Repressor Exerts Tighter Control on Its Cognate Promoter Than the Escherichia coli System.
UniProt entry P37309 (ARSR_ECOLI)