Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
Floral transcription factor AGAMOUS interacts in vitro with a leucine-rich repeat and an acid phosphatase protein complex.
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AG interacts with the FLOR1-VSP1 complex and directly with FLOR1 in vitro.
"Coimmunoprecipitation, two-hybrid yeast, and affinity column assays show that the FLOR1-VSP1 complex interacts with AGAMOUS and that this transcription factor directly interacts with FLOR1."
Molecular and phylogenetic analyses of the complete MADS-box transcription factor family in Arabidopsis: new openings to the MADS world.
MADS-box protein complexes control carpel and ovule development in Arabidopsis.
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AG is necessary for stamen and carpel development.
"The AGAMOUS (AG) gene is necessary for stamen and carpel development"
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AG forms SEP-dependent multimeric MADS-box complexes.
"We also show that STK, AG, SHP1, and SHP2 can form multimeric complexes and that these interactions require the SEPALLATA (SEP) MADS-box proteins."
Defining subdomains of the K domain important for protein-protein interactions of plant MADS proteins.
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Cached abstract describes K-domain interaction studies for AP3/PI/SEP proteins but does not expose AG-specific interaction evidence.
"Here we report on our studies on the interactions of the B class MADS proteins AP3 and PI with the E class MADS proteins SEP1, SEP2, and SEP3."
Transcriptional program controlled by the floral homeotic gene AGAMOUS during early organogenesis.
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AG terminates meristem activity and promotes stamen and carpel development.
"In Arabidopsis, the homeotic gene AGAMOUS (AG) terminates meristem activity and promotes development of stamens and carpels."
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AG targets were validated by expression and binding assays.
"Novel targets were validated by in situ hybridisation and binding to AG in vitro and in vivo."
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AG participates in a positive-feedback transcriptional loop with other floral homeotic proteins.
"The results also suggest that AG and other homeotic proteins with which it interacts (SEPALLATA3, APETALA3, PISTILLATA) are coordinately regulated in a positive-feedback loop to maintain their own expression"
Comprehensive interaction map of the Arabidopsis MADS Box transcription factors.
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Arabidopsis MADS-box proteins form specific heterodimers and some homodimers.
"A matrix-based yeast two-hybrid screen of >100 members of this family revealed a collection of specific heterodimers and a few homodimers."
The protein encoded by the Arabidopsis homeotic gene agamous resembles transcription factors.
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AG probably encodes a transcription factor regulating stamen and carpel development.
"The agamous gene therefore probably encodes a transcription factor that regulates genes determining stamen and carpel development in wild-type flowers."
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agamous mutations transform Arabidopsis floral sex organs.
"Mutations in the homeotic gene agamous of the plant Arabidopsis cause the transformation of the floral sex organs."
A single amino acid change in the enhancer of zeste ortholog CURLY LEAF results in vernalization-independent, rapid flowering in Arabidopsis.
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clf-59 has no noticeable effect on AG, while CLF loss of function derepresses AG among other genes.
"clf-59 causes hyperrepression of FLC expression with no noticeable effect on AG."
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CLF loss of function derepresses AG, supporting a Polycomb/ectopic expression context rather than a normal leaf-development role for AG.
"In contrast, clf loss-of function mutants cause derepression of several genes, including AG, FLC, and FT"
Characterization of MADS-domain transcription factor complexes in Arabidopsis flower development.
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AG-containing floral MADS proteins interact in floral tissues as predicted by the floral quartet model.
"five major floral homeotic MADS-domain proteins (AP1, AP3, PI, AG, and SEP3) interact in floral tissues as proposed in the "floral quartet" model."
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Heteromeric MADS-domain protein complexes coexist within the nucleus.
"Based on our in vitro EMSA studies, we propose that different heteromeric MADS-domain protein complexes can coexist within the nucleus"
At-MINI ZINC FINGER2 and Sl-INHIBITOR OF MERISTEM ACTIVITY, a Conserved Missing Link in the Regulation of Floral Meristem Termination in Arabidopsis and Tomato.
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AG directly represses WUS during early floral meristem termination.
"First, AG directly represses the WUS locus during the early stages of FM termination (stage 3)"
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AG binding to WUS recruits Polycomb repression machinery.
"The binding of AG to the WUS locus mediates the recruitment of CURLY LEAF, a core component of the Polycomb Repressive Complex 2 (PRC2)"
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AG directly induces KNU as part of meristem termination.
"The expression of At KNU is directly induced by AG at stages 5 and 6"
The protein-protein interaction landscape of transcription factors during gynoecium development in Arabidopsis.
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The study produced a broad gynoecium TF PPI map but cached text does not document the specific AG interaction row.
"We analyzed almost 4500 combinations and detected more than 250 protein-protein interactions (PPIs), resulting in a process-specific interaction map."
DNA-binding properties of Arabidopsis MADS domain homeotic proteins APETALA1, APETALA3, PISTILLATA and AGAMOUS.
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AG homocomplexes bind CArG-box sequences, supporting sequence-specific regulatory DNA binding.
"AP1 and AG homocomplexes and AP3-PI heterocomplexes bind to CArG-box sequences."
Determination of Arabidopsis floral meristem identity by AGAMOUS.
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AG is required for final floral meristem identity.
"AG function is required for the final definition of floral meristem identity"
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AG controls the transition from indeterminate to determinate meristem activity.
"AG is a principal developmental switch that controls the transition of meristem activity from indeterminate to determinate."
Specific interactions between the K domains of AG and AGLs, members of the MADS domain family of DNA binding proteins.
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The AG K domain binds K domains of AGL proteins.
"Additional analysis showed that the K domain of AG alone was able to bind the K domains of these AGLs."
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AG interacts with AGL2, AGL4, AGL6, and AGL9.
"These results strongly suggest that AG interacts with AGL2, AGL4, AGL6 and AGL9 in vivo."
UniProtKB entry P17839 for Arabidopsis AGAMOUS
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UniProt describes AG as a floral homeotic protein required for stamen/carpel development and meristem determinacy.
"Probable transcription factor involved in the control of organ identity during the early development of flowers. Is required for normal development of stamens and carpels in the wild-type flower. Plays a role in maintaining the determinacy of the floral meristem."
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UniProt records AG homodimerization, CArG binding, and heterodimerization with SEPALLATA/AGL proteins.
"SUBUNIT: Homodimer, capable of binding to CArG-box sequences. Forms a heterodimer via the K-box domain with either SEPALATTA1/AGL2, SEPALATTA2/AGL4, SEPALLATA3/AGL9 or AGL6."
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UniProt records AG as nuclear.
"SUBCELLULAR LOCATION: Nucleus."
Falcon deep research report for Arabidopsis AG
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AG is the Arabidopsis C-class MIKC-type MADS-domain transcription factor.
"AGAMOUS (AG; P17839; At4g18960) encodes a MIKC-type MADS-domain transcription factor acting as the canonical C-function floral homeotic regulator in Arabidopsis."
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Recent literature supports SEP-driven AG-containing tetramerization in inner-whorl organ identity and determinacy.
"SEP3-driven tetramerization with AG is required not only for meristem determinacy but also for inner-whorl organ identity (whorls 2-4)."
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AG participates in floral meristem determinacy by repressing WUS and engaging KNU/CRC-associated pathways.
"A core pathway-level function of AG is termination of floral stem cell maintenance by repressing the stem-cell regulator WUSCHEL (WUS)."
PANTHER family metadata for PTHR48019
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PTHR48019 is the MADS-box/MEF2 transcription factor family and provides family context for AG, but its LLM-generated family description was not treated as primary evidence.
"name: MADS-box/MEF2 Transcription Factor"
Complexes of MADS-box proteins are sufficient to convert leaves into floral organs.
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AG joins B-class and A-class floral homeotic complexes through SEP3, and PI/AP3/SEP3/AG together convert leaves into staminoid organs.
"leaves of triply transgenic plants, 35S::PI;35S::AP3;35S::AP1 or 35S::PI;35S::AP3;35S::SEP3, are transformed into petaloid organs and that those of 35S::PI;35S::AP3;35S::SEP3;35S::AG are transformed into staminoid organs"